Liang Wenlong, Xie Zhengang, Cheng Jingli, Xiao Douxin, Xiong Qiuyu, Wang Qiangwei, Zhao Jinhao, Gui Wenjun
Institute of Pesticide and Environmental Toxicology, Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou 310058, P.R. China.
ACS Nano. 2021 Apr 27;15(4):6987-6997. doi: 10.1021/acsnano.0c10877. Epub 2021 Apr 15.
Using a simple one-pot method, we developed a prochloraz (Pro) and pH-jump reagent-loaded zeolitic imidazolate framework-8 (PD@ZIF-8) composite for the smart control of Sclerotinia disease. The pH-jump reagent can induce the acidic degradation of ZIF-8 using UV light to realize the controlled release of Pro. Thus, the physical properties of PD@ZIF-8, such as its release, formulation stability, and adhesion, were investigated in detail. The results showed that the quantity of Pro released by PD@ZIF-8 under UV light irradiation (365 nm) was 63.4 ± 3.5%, whereas under dark conditions, it was only 13.7 ± 0.8%. activity indicated that the EC of PD@ZIF-8 under UV light irradiation was 0.122 ± 0.02 μg/mL, which was not significantly different from that of Pro (0.107 ± 0.01 μg/mL). Pot experiments showed that the efficacy of PD@ZIF-8 under light irradiation was 51.2 ± 5.7% for a fungal infection at 14 days post-spraying, whereas the effectiveness of prochloraz emulsion in water was only 9.3 ± 3.3%. Furthermore, fluorescence tracking of ZIF-8 and biosafety experiments showed that ZIF-8 could be absorbed by plant leaves and transported to various parts of oilseed rape in a short period of time and that PD@ZIF-8 was relatively safe for plants and HepG2 cells. These results highlight the potential of the composite to provide efficient and smart delivery of fungicides into plants for protection against diseases and provide an idea for developing sustainable agriculture.
我们采用简单的一锅法，制备了负载咪鲜胺（Pro）和pH跃变试剂的沸石咪唑酯骨架材料-8（PD@ZIF-8）复合材料，用于智能防控菌核病。pH跃变试剂可利用紫外光诱导ZIF-8发生酸性降解，从而实现咪鲜胺的控释。因此，我们详细研究了PD@ZIF-8的物理性质，如释放性能、制剂稳定性和附着力。结果表明，PD@ZIF-8在紫外光（365 nm）照射下释放的咪鲜胺量为63.4±3.5%，而在黑暗条件下仅为13.7±0.8%。活性表明，PD@ZIF-8在紫外光照射下的EC为0.122±0.02 μg/mL，与咪鲜胺（0.107±0.01 μg/mL）无显著差异。盆栽试验表明，喷雾后14天，PD@ZIF-8在光照下对真菌感染的防治效果为51.2±5.7%，而水基咪鲜胺乳油的防治效果仅为9.3±3.3%。此外，ZIF-8的荧光追踪和生物安全性实验表明，ZIF-8可被植物叶片吸收，并在短时间内运输到油菜的各个部位，且PD@ZIF-8对植物和HepG2细胞相对安全。这些结果突出了该复合材料在为植物提供高效、智能的杀菌剂递送以防治病害方面的潜力，并为发展可持续农业提供了思路。
