Industry 4.0 Convergence Bionics Engineering, Pukyong National University, Busan 48513, Republic of Korea.
New-senior Healthcare Innovation Center (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea.
Int J Biol Macromol. 2021 Jul 31;183:55-67. doi: 10.1016/j.ijbiomac.2021.04.053. Epub 2021 Apr 20.
In recent years, suitable bioactive materials coated nanoparticles have attracted substantial attention in the field of biomedical applications. The present study emphasizes experimental details for the synthesis of boiling rice starch extract (BRE) coated iron oxide nanoparticles (IONPs) to treat cancer by photoacoustic imaging (PAI)-guided chemo-photothermal therapy. The solvothermal method was used to synthesize IONPs. The physical immobilization method helps to coat BRE-loaded doxorubicin (DOX) molecules on the iron oxide surface. In vitro drug release was estimated in basic (pH 9.0), neutral (pH 7.2), and acidic (pH 4.5) media for varying time periods using ultraviolet-visible spectroscopy. The chemical and physical properties of the synthesized spherical BRE-IONPs were characterized using sophisticated analytical instrumentation. A magnetic saturation experiment was performed with BRE-IONPs for evaluating possible hyperthermia in targeted drug delivery. The biological activity of the synthesized BRE-IONPs was investigated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and acridine orange/propidium iodide fluorescence cell viability study. BRE-IONPs showed excellent photothermal stability, with a high photothermal conversion efficiency (η = 29.73%), biocompatible property, and high near-infrared region absorption for PAI-guided PTT treatment. This study will provide a better understanding of rice starch as a suitable bioactive coating material for possible theranostic applications.
近年来，合适的生物活性材料包覆的纳米粒子在生物医学应用领域引起了广泛关注。本研究强调了通过光声成像（PAI）引导的化学-光热治疗来治疗癌症的沸腾米淀粉提取物（BRE）包覆氧化铁纳米粒子（IONPs）的合成实验细节。采用溶剂热法合成 IONPs。物理固定化方法有助于将 BRE 负载的阿霉素（DOX）分子涂覆在氧化铁表面。通过紫外可见光谱法在不同时间间隔的碱性（pH 9.0）、中性（pH 7.2）和酸性（pH 4.5）介质中估算体外药物释放。使用先进的分析仪器对合成的球形 BRE-IONPs 的化学和物理性质进行了表征。通过 BRE-IONPs 进行了磁饱和实验，以评估在靶向药物输送中可能发生的热疗。通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴化物测定法和吖啶橙/碘化丙啶荧光细胞活力研究评估了合成的 BRE-IONPs 的生物活性。BRE-IONPs 表现出优异的光热稳定性，具有高光热转换效率（η=29.73%）、生物相容性和高近红外区域吸收，可用于 PAI 引导的 PTT 治疗。本研究将为米淀粉作为一种合适的生物活性涂层材料用于潜在的治疗应用提供更好的理解。
