Department of Plastic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, People's Republic of China.
Zhejiang University School of Medicine, Hangzhou, 310000, People's Republic of China.
Biomed Eng Online. 2022 Jan 11;21(1):2. doi: 10.1186/s12938-022-00976-x.
The role of epithelial-mesenchymal transition (EMT) in the pathogenesis of keloids is currently raising increasing attention. Long noncoding RNAs (lncRNAs) govern a variety of biological processes, such as EMT, and their dysregulation is involved in many diseases including keloid disease. The aim of this study was to identify differentially expressed EMT-related lncRNAs in keloid tissues versus normal tissues and to interpret their functions.
Eleven lncRNAs and 16 mRNAs associated with EMT were identified to have differential expression between keloid and normal skin tissues (fold change > 1.5, P < 0.05). Gene Ontology (GO) analysis showed that these differentially expressed mRNAs functioned in the extracellular matrix, protein binding, the positive regulation of cellular processes, the Set1C/COMPASS complex and histone acetyltransferase activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that these mRNAs are involved in pathways in cancer. The lncRNA, XLOC_000587 may promote cell proliferation and migration by enhancing the expression of ENAH, while AF268386 may facilitate the invasive growth of keloids by upregulating DDR2.
We characterized the differential expression profiles of EMT-related lncRNAs and mRNAs in keloids, which may contribute to preventing the occurrence and development of keloids by targeting the corresponding signaling pathways. These lncRNAs and mRNAs may provide biomarkers for keloid diagnosis and serve as potential targets for the treatment of this disease.
上皮-间充质转化（EMT）在瘢痕疙瘩发病机制中的作用目前受到越来越多的关注。长链非编码 RNA（lncRNA）调控多种生物学过程，如 EMT，其失调与包括瘢痕疙瘩疾病在内的许多疾病有关。本研究旨在鉴定瘢痕疙瘩组织与正常组织中差异表达的 EMT 相关 lncRNA，并解释其功能。
鉴定出 11 个 lncRNA 和 16 个与 EMT 相关的 mRNAs 在瘢痕疙瘩和正常皮肤组织之间存在差异表达（倍数变化>1.5，P<0.05）。基因本体论（GO）分析表明，这些差异表达的 mRNAs 在细胞外基质、蛋白结合、细胞过程的正调控、Set1C/COMPASS 复合物和组蛋白乙酰转移酶活性中发挥作用。京都基因与基因组百科全书（KEGG）分析表明，这些 mRNAs参与癌症相关途径。lncRNA XLOC_000587 可能通过增强 ENAH 的表达促进细胞增殖和迁移，而 AF268386 可能通过上调 DDR2 促进瘢痕疙瘩的侵袭性生长。
我们描述了瘢痕疙瘩中 EMT 相关 lncRNA 和 mRNAs 的差异表达谱，这些差异表达可能通过靶向相应的信号通路有助于预防瘢痕疙瘩的发生和发展。这些 lncRNA 和 mRNAs 可为瘢痕疙瘩的诊断提供生物标志物，并可能成为治疗这种疾病的潜在靶点。
