College of Animal & Veterinary Sciences, Southwest Minzu Universitygrid.412723.1, Chengdu, China.
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization Chengdu, China.
Microbiol Spectr. 2022 Aug 31;10(4):e0121822. doi: 10.1128/spectrum.01218-22. Epub 2022 Jul 7.
A total of 232 goat fecal samples (124 diarrheic and 108 nondiarrheic) collected from 12 farms in Southwest China were tested for astrovirus using RT-PCR. A total of 16.9% (21/124) of diarrheic and 20.4% (22/108) of nondiarrheic samples were astrovirus-positive, and no statistical difference was found in the detection rate between healthy and sick goats. Furthermore, 28 obtained complete ORF2 sequences could be classified into six genotypes according to the species classification criteria of the International Committee on Taxonomy of Viruses (ICTV). It is worth noting that, in addition to four known caprine astrovirus genotypes (, , , and ), and genotypes were identified in goats. Interestingly, five of 19 ORF2 sequences in the genotype showed possible intragenotypic recombination events. Furthermore, nearly complete caprine astrovirus genomes of and genotypes were obtained. The genome of the SWUN/ECJK3/2021 strain shared the highest similarity (62.0% to 73.9%) with astrovirus in , and clustered in the so-called human-mink-ovine (HMO) clade, which contained the majority of the neurotropic astrovirus strains. Moreover, the SWUN/LJK2-2/2020 strain showed the highest similarity (69.7% to 78.6%) and the closest genetic relationship to the known porcine and bovine astroviruses in . In conclusion, this study confirmed six genotypes of astrovirus circulating among goats in Southwest China, including and genotypes. These findings enhance our knowledge of the prevalence and diversity of astroviruses. Caprine astrovirus is a newly emerging virus, and information regarding its prevalence and molecular characteristics remains limited. In this study, six genotypes of astrovirus, including and , were identified in goats, adding two novel caprine astrovirus genotypes to the four previously known genotypes, thereby enriching the diversity of the caprine astrovirus. Moreover, genomes of SWUN/ECJK3/2021 and SWUN/LJK2-2/2020 strains were obtained from goats, which aids in the understanding of the infection spectrum and host range of the two genotypes. This study is the first to demonstrate the presence of neurotropic-like astrovirus () in goats, which has significant implications for the diagnosis of neurological diseases in goats.
从中国西南地区的 12 个农场采集了 232 份山羊粪便样本（124 份腹泻和 108 份非腹泻），使用 RT-PCR 检测星状病毒。在腹泻样本中，16.9%（21/124）和非腹泻样本中，20.4%（22/108）的样本为星状病毒阳性，健康和患病山羊的检测率无统计学差异。此外，从 28 个获得的完整 ORF2 序列可以根据病毒分类国际委员会（ICTV）的物种分类标准分为六个基因型。值得注意的是，除了四种已知的山羊星状病毒基因型（、、和）外，在山羊中还鉴定出了和基因型。有趣的是，在基因型中，19 个 ORF2 序列中的 5 个可能存在基因内重组事件。此外，还获得了和基因型的近乎完整的山羊星状病毒基因组。SWUN/ECJK3/2021 株的基因组与星状病毒的同源性最高（62.0%至 73.9%），聚类在所谓的人类-貂-羊（HMO）分支中，该分支包含大多数神经毒性星状病毒株。此外，SWUN/LJK2-2/2020 株与已知的猪和牛星状病毒的相似度最高（69.7%至 78.6%），遗传关系最密切。总之，本研究证实了在中国西南地区山羊中流行的六种星状病毒基因型，包括和基因型。这些发现增强了我们对星状病毒流行和多样性的认识。山羊星状病毒是一种新出现的病毒，其流行情况和分子特征的信息仍然有限。在本研究中，从山羊中鉴定出了六种星状病毒基因型，包括和基因型，为之前已知的四种基因型增加了两种新型山羊星状病毒基因型，丰富了山羊星状病毒的多样性。此外，从山羊中获得了 SWUN/ECJK3/2021 和 SWUN/LJK2-2/2020 株的基因组，有助于了解这两种基因型的感染谱和宿主范围。本研究首次证明了神经毒性样星状病毒（）在山羊中的存在，这对山羊神经疾病的诊断具有重要意义。
