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两种快速方法用于鉴定常见非发酵或氧化酶阳性革兰氏阴性杆菌的评估。

Evaluation of two rapid methods for identification of commonly encountered nonfermenting or oxidase-positive, Gram-negative rods.

作者信息

Dowda H

出版信息

J Clin Microbiol. 1977 Dec;6(6):605-9. doi: 10.1128/jcm.6.6.605-609.1977.

DOI:10.1128/jcm.6.6.605-609.1977
PMID:338626
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC274832/
Abstract

The ability of the expanded API-20E and the Oxi-Ferm System to identify 176 isolates of nonfermenting or oxidase-positive, gram-negative rods from 17 species or groups was studied. The expanded API-20E, without referral to a computer reference center, was able to identify 61.4% of the isolates to the species level. If reference to the computer center was utilized, an additional 25% could be identified. Of the isolates examined, 13.6% were misidentified, partially identified, or assigned no identification. Those assigned to the incorrect genus constituted 0.5% of the isolates tested; those assigned to the correct genus, but the wrong species, constituted 0.5%; 4.5% were placed in the correct genus with no species given; and 7.9% were assigned no identification. The Oxi-Ferm System was able to assign 75% of the isolates to the correct species without further testing, and an additional 19.3% required additional testing for correct identification. Those assigned to the incorrect genus represented 3.4% of the isolates tested; 1.1% were assinged to the correct genus, but the wrong species; and 1.1% were assigned to the correct genus, with no species indicated.

摘要

对扩展API - 20E和Oxi - Ferm系统鉴定来自17个物种或类群的176株非发酵或氧化酶阳性革兰氏阴性杆菌的能力进行了研究。扩展API - 20E在不参考计算机参考中心的情况下,能够将61.4%的菌株鉴定到种水平。如果利用计算机中心,则可再鉴定出25%。在所检查的菌株中,13.6%被错误鉴定、部分鉴定或未给出鉴定结果。被归为错误属的占所测试菌株的0.5%;被归为正确属但错误种的占0.5%;4.5%被置于正确属但未给出种名;7.9%未给出鉴定结果。Oxi - Ferm系统无需进一步测试就能将75%的菌株鉴定到正确种,另外19.3%需要进一步测试才能正确鉴定。被归为错误属的占所测试菌株的3.4%;1.1%被归为正确属但错误种;1.1%被归为正确属但未指明种名。

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