用于鉴定葡萄糖非发酵革兰氏阴性杆菌的自动微生物系统评估。
Evaluation of the AutoMicrobic system for identification of glucose-nonfermenting gram-negative rods.
作者信息
Smith S M, Cundy K R, Gilardi G L, Wong W
出版信息
J Clin Microbiol. 1982 Feb;15(2):302-7. doi: 10.1128/jcm.15.2.302-307.1982.
Abstract
With the Enterobacteriaceae Plus Biochemical Card, the AutoMicrobic system (Vitek Systems, Inc., Hazelwood, Mo.) is purported to identify members of the family Enterobacteriaceae and seven species of glucose-nonfermenting gram-negative rods: Pseudomonas aeruginosa, P. fluorescens, P. putida, P. maltophilia, P. cepacia, and Acinetobacter calcoaceticus (saccharolytic and non-saccharolytic). The latter capability was examined in this investigation. Of 410 glucose-nonfermenting rods included in the identification profile, the AutoMicrobic system correctly identified 366 (89.3%). Of 62 glucose-nonfermenting organisms not included in the identification profile, 41 (66%) were correctly reported as "unidentified organism." The usefulness of the AutoMicrobic system-generated identification probability in establishing criteria for acceptance of identification is discussed.
摘要
使用肠杆菌科细菌生化鉴定卡时，自动微生物分析系统（Vitek系统公司，密苏里州黑兹尔伍德）据称可鉴定肠杆菌科细菌成员以及7种不发酵葡萄糖的革兰氏阴性杆菌：铜绿假单胞菌、荧光假单胞菌、恶臭假单胞菌、嗜麦芽窄食单胞菌、洋葱伯克霍尔德菌以及醋酸钙不动杆菌（分解糖型和非分解糖型）。本研究对该系统的后一项功能进行了检测。在鉴定谱中包含的410株不发酵葡萄糖的杆菌中，自动微生物分析系统正确鉴定出366株（89.3%）。在鉴定谱中未包含的62株不发酵葡萄糖的微生物中，有41株（66%）被正确报告为“未鉴定出的微生物”。本文还讨论了自动微生物分析系统生成的鉴定概率在确立鉴定可接受标准方面的实用性。
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