School of Energy and Power Engineering, Jiangsu University, Jiangsu 212013, China.
School of Energy and Power Engineering, Jiangsu University, Jiangsu 212013, China.
Bioresour Technol. 2021 Jul;332:125118. doi: 10.1016/j.biortech.2021.125118. Epub 2021 Apr 5.
The phytohormone producing Streptomyces rosealbus MTTC 12,951 (S.R) and green microalga Chlorella vulgaris MSU-AGM 14 (C.V) were cultivated in co-culture system to evaluate exogenous hormonal activity. Biosynthesis of indole-3-acetic acid (IAA) and their precursors were quantitatively evaluated by employing High Performance Liquid Chromatography (HPLC). The concentration of IAA (0.72 ± 0.02 µg mL) was observed to be elevated in co-cultivation system due to symbiotic interaction between Streptomyces and microalgae. In exchange, microalgae produced adequate volume of tryptophan (Trp) to induce IAA biosynthesis. The Trp stress in late exponential phase encouraged lipid accumulation (175 ± 10 mg g). The bioflocculation property of microalgae ensures potential and economic viable harvesting process by reducing 148% input energy compared to conventional method. The overall results evidenced that C.V co-cultivation with S.R exhibits promotional behavior and serves as a promising cultivation process for microalgae in terms of cost efficiency and energy conservation.
植物激素产生菌玫瑰色链霉菌 MTTC 12,951（S.R）和绿色微藻普通小球藻 MSU-AGM 14（C.V）在共培养系统中培养，以评估外源激素活性。采用高效液相色谱法（HPLC）定量评估了吲哚-3-乙酸（IAA）及其前体的生物合成。由于链霉菌和微藻之间的共生相互作用，共培养系统中观察到 IAA（0.72±0.02µg mL）浓度升高。作为交换，微藻产生了足够量的色氨酸（Trp）来诱导 IAA 生物合成。在指数后期，Trp 胁迫促进了脂质积累（175±10mg g）。微藻的生物絮凝特性通过减少与传统方法相比 148%的输入能量，确保了潜在的经济可行的收获过程。总体结果表明，C.V 与 S.R 的共培养表现出促进作用，并且在成本效益和节能方面是微藻培养的有前途的过程。
