Bagheri Parisa, Sharifi Mohammadreza, Ghadiri Ava
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Science, Isfahan, 81744-176 Iran.
Indian J Hematol Blood Transfus. 2021 Apr;37(2):232-239. doi: 10.1007/s12288-020-01324-6. Epub 2020 Jul 24.
Long noncoding ribonucleic acids (lncRNAs) are ribonucleic acid (RNA) molecules longer than 200 nucleotides without protein-coding capacity. Several studies have shown that lncRNAs play a pivotal role in the initiation, maintenance, and progression of acute myeloid leukemia (AML), which could make them a promising candidate in the diagnosis and treatment of leukemia. Acute Megakaryoblastic leukemia (AMKL) is a rare form of AML with a poor prognosis and low survival. It has been reported that lncRNA MIR100HG is involved several types of malignancies. In the present study, MIR100HG was downregulated in a human acute megakaryoblastic leukemia cell line (M-07e) using Antisense LNA GapmeRs. In order to assess the expression level of MIR100HG, cell viability, apoptosis, and necrosis (late apoptosis), quantitative reverse transcription polymerase chain reaction (qRT-PCR), Methyl-thiazol Tetrazolium assay, AnnexinV, and propidium iodide staining was performed at different time points after the transfection. In addition, the expression level of TGFβ was evaluated by qRT-PCR. Our results revealed that inhibition of MIR100HG might serve as a new method for inhibition of the proliferation of AMKL cells and therefore, could be a promising approach in medicine for targeted therapy in AMKL.
长链非编码核糖核酸（lncRNAs）是长度超过200个核苷酸且无蛋白质编码能力的核糖核酸（RNA）分子。多项研究表明，lncRNAs在急性髓系白血病（AML）的发生、维持和进展中起关键作用，这使其有望成为白血病诊断和治疗的候选靶点。急性巨核细胞白血病（AMKL）是AML的一种罕见形式，预后较差，生存率低。据报道，lncRNA MIR100HG与多种恶性肿瘤有关。在本研究中，使用反义锁核酸GapmeRs在人急性巨核细胞白血病细胞系（M-07e）中下调MIR100HG。为了评估MIR100HG的表达水平、细胞活力、凋亡和坏死（晚期凋亡），在转染后的不同时间点进行了定量逆转录聚合酶链反应（qRT-PCR）、甲基噻唑四氮唑法、膜联蛋白V和碘化丙啶染色。此外，通过qRT-PCR评估TGFβ的表达水平。我们的结果表明，抑制MIR100HG可能是抑制AMKL细胞增殖的一种新方法，因此，可能是AMKL靶向治疗的一种有前景的医学方法。
