来自植物内生真菌的新型生物活性倍半萜类化合物

Liu Gaoran, Huo Ruiyun, Zhai Yanan, Liu Ling

State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.

Front Microbiol. 2021 Mar 31;12:641504. doi: 10.3389/fmicb.2021.641504. eCollection 2021.

Three new secondary metabolites pestalothenins A-C (-), including two new humulane-derived sesquiterpeniods ( and ) and one new caryophyllene-derived sesquiterpeniod (), together with five known compounds (-) were isolated from the crude extract of the plant endophytic fungus (N635). Their structures were elucidated by the extensive analyses of HRESIMS and NMR spectroscopic data. The absolute configurations of - were determined by comparison of experimental and calculated electronic circular dichroism (ECD) spectra. The cytotoxic effects of these compounds were evaluated . Compound showed moderate cytotoxicity against T24 and MCF7 cell lines. In addition, compounds - were also evaluated for antibacterial activity.

从植物内生真菌（N635）的粗提物中分离出三种新的次生代谢产物——pestalothenins A-C（-），包括两种新的源自葎草烷的倍半萜类化合物（和）以及一种新的源自石竹烯的倍半萜类化合物（），同时还分离出五种已知化合物（-）。通过对高分辨电喷雾电离质谱（HRESIMS）和核磁共振（NMR）光谱数据的广泛分析阐明了它们的结构。通过比较实验和计算的电子圆二色光谱（ECD）确定了 - 的绝对构型。评估了这些化合物的细胞毒性作用。化合物对T24和MCF7细胞系表现出中等细胞毒性。此外，还评估了化合物 - 的抗菌活性。