Khan Ikram, Prabhakar Anil, Delepine Chloe, Tsang Hayley, Pham Vincent, Sur Mriganka
Department of Electrical Engineering, Indian Institute of Technology, Madras 600036, India.
Picower Institute for Learning and Memory, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Biomicrofluidics. 2021 Apr 6;15(2):024105. doi: 10.1063/5.0041027. eCollection 2021 Mar.
Organoids are biological systems grown and are observed to self-organize into 3D cellular tissues of specific organs. Brain organoids have emerged as valuable models for the study of human brain development in health and disease. Researchers are now in need of improved culturing and imaging tools to capture the dynamics of development processes in the brain. Here, we describe the design of a microfluidic chip and bioreactor, to enable tracking and imaging of brain organoids on-chip. The low-cost 3D printed microfluidic bioreactor supports organoid growth and provides an optimal imaging chamber for live-organoid imaging, with drug delivery support. This fully isolated design of a live-cell imaging and culturing platform enables long-term live-imaging of the intact live brain organoids as it grows. We can thus analyze their self-organization in a controlled environment with high temporal and spatial resolution.
类器官是培养而成的生物系统，并且观察发现它们能自组织形成特定器官的三维细胞组织。脑类器官已成为研究健康和疾病状态下人类大脑发育的重要模型。研究人员目前需要改进的培养和成像工具，以捕捉大脑发育过程的动态变化。在此，我们描述了一种微流控芯片和生物反应器的设计，以实现对芯片上脑类器官的追踪和成像。这种低成本的3D打印微流控生物反应器支持类器官生长，并为活类器官成像提供了一个最佳成像室，同时具备药物递送支持。这种活细胞成像和培养平台的完全隔离设计能够在完整的活脑类器官生长过程中对其进行长期实时成像。因此，我们可以在可控环境中以高时空分辨率分析它们的自组织过程。
