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在饲料中使用发酵鸡羽毛粉来提高银鲳(Lacepede, 1801)的消化酶活性和营养消化率。

Use of chicken feather meal fermented with in diets to increase the digestive enzymes activity and nutrient digestibility of silver pompano (Lacepede, 1801).

机构信息

Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Riau, Pekanbaru, 28293, Indonesia.

Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Riau, Pekanbaru, 28293, Indonesia.

出版信息

F1000Res. 2021 Jan 15;10:25. doi: 10.12688/f1000research.26834.2. eCollection 2021.

Abstract

: Feather has the potential to be used as a fish feed ingredient because it has high protein content (80-85%), and is rich in amino acids arginine, leucine, isoleucine and valine. However, the protein consists mainly of keratin, which is classified as fiber that is difficult to digest. Therefore, to improve digestibility, the keratin protein is degraded using microbial . This study aimed to determine the digestibility of fermented feather meal (FFM) in silver pompano ( ) diets and to observe the histological structure of their intestines after digestion. : The method used was a one factor experiment with five treatments and three replications each, which were: diet without FFM, diet containing 10% FFM, 20%, 30% and 40%. The diets were given to juvenile silver pompano (with average body weight of 8.56 ± 0.18 g) and stocked in 15 similar 20-L plastic jars with 10 fish per jar in a density of 100 L capacity container. The experimental diets were given three times daily at approximately 8.00 AM, 12.00 PM and 5.00 PM to apparent satiation for 60 days. : The results showed that the use of FFM increased the activity of digestive enzymes (protease and lipase), but reduced the amylase activity of silver pompano, which was significantly different between treatments (P <0.05). Meanwhile, the diet containing 20% FFM produced the highest feed and protein, which are 37.05% and 67.24%, respectively. This was significantly different from other treatments (P <0.05), and was effectively absorbed by fish intestines. The addition of chicken feather meal fermented with could increase the activity of protease and lipase enzymes and nutrient digestibility of silver pompano but not amylase activity.

摘要

羽毛具有作为鱼饲料成分的潜力,因为它的蛋白质含量高(80-85%),并且富含精氨酸、亮氨酸、异亮氨酸和缬氨酸等氨基酸。然而,蛋白质主要由角蛋白组成,角蛋白被归类为难以消化的纤维。因此,为了提高消化率,角蛋白蛋白使用微生物降解。本研究旨在确定发酵羽毛粉(FFM)在银鲳()饲料中的消化率,并观察其消化后的肠道组织学结构。:本研究采用单因素实验设计,设有 5 个处理组,每个处理组设 3 个重复,分别为:不含 FFM 的饲料、含 10%FFM、20%、30%和 40%FFM 的饲料。将饲料喂给平均体重为 8.56 ± 0.18 g 的幼龄银鲳,并将 10 条鱼装在 15 个 20-L 塑料罐中,每个罐中密度为 100 L 容量容器的 10 条鱼。实验饲料每天分 3 次投喂,大约在上午 8.00 点、中午 12.00 点和下午 5.00 点,以满足明显的饱食感,共 60 天。:结果表明,FFM 的使用提高了消化酶(蛋白酶和脂肪酶)的活性,但降低了银鲳的淀粉酶活性,处理之间存在显著差异(P <0.05)。同时,含 20%FFM 的饲料产生了最高的饲料和蛋白质利用率,分别为 37.05%和 67.24%。这与其他处理有显著差异(P <0.05),并且被鱼肠有效吸收。用发酵鸡羽毛粉添加可以增加银鲳的蛋白酶和脂肪酶酶活性和营养物质消化率,但不增加淀粉酶活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1287/8030641/f0c20f2e6be5/f1000research-10-55545-g0000.jpg

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