Kamaraj Sathvika, Vuppu Suneetha
School of Biosciences and Technology, Department of Biotechnology, VIT University, Vellore, Tamil Nadu, 632014, India.
Mol Biotechnol. 2023 Sep 16. doi: 10.1007/s12033-023-00862-4.
Products produced from waste are a relatively recent innovation. Feather substrates are abundant in keratin content and improper disposal can cause ecosystem contamination. However, these pollutants can be transformed into value-added products for industrial application. Physical, chemical and cutting-edge microbiological methods were utilized for decomposing keratin and aid in the identification and estimation of amino acids from poultry feather wastes. These beneficial approaches are receiving more attention due to their retrieval of harmless and value added byproducts. These keratin-based compounds are used widely in pharmaceutical, livestock feed, fertilizer, and a variety of other industrial sectors. Since keratin is primarily consisting of amino acids, it can be utilized to affirm and estimate the amino acids in these feather substrates. This study primarily highlights the various methodologies employed for the qualitative estimation of amino acids in feather waste samples and the inhibitory activity of keratinase enzyme by EDTA and pepstatin in order to accumulate amino acids for drug delivery purpose and their importance in various pharmaceutical industries. In addition to that, molecular docking studies of cysteine with many standard pharmaceutical drugs like acetaminophen, pethidine, methylphenidate, carbamazepine, cillin and amlodipine were performed using autodock to demonstrate how cysteine greatly reduces conventional drug toxicity and its side effects.
利用废弃物生产产品是一项相对较新的创新。羽毛底物富含角蛋白，不当处理会导致生态系统污染。然而，这些污染物可转化为具有工业应用价值的增值产品。采用物理、化学和前沿微生物学方法分解角蛋白，并有助于从家禽羽毛废弃物中鉴定和估算氨基酸。这些有益的方法因其能回收无害且增值的副产品而受到更多关注。这些基于角蛋白的化合物广泛应用于制药、家畜饲料、肥料及其他各种工业领域。由于角蛋白主要由氨基酸组成，它可用于确认和估算这些羽毛底物中的氨基酸。本研究主要强调了用于羽毛废弃物样品中氨基酸定性估算的各种方法，以及EDTA和胃蛋白酶抑制剂对角蛋白酶的抑制活性，以便为药物递送目的积累氨基酸及其在各种制药行业中的重要性。此外，使用自动对接软件对半胱氨酸与对乙酰氨基酚、哌替啶、哌甲酯、卡马西平、西林和氨氯地平等多种标准药物进行分子对接研究，以证明半胱氨酸如何极大地降低传统药物的毒性及其副作用。
