Zubair Muhammad Sulaiman, Khairunisa Siti Qamariyah, Widodo Agustinus, Pitopang Ramadanil
Department of Pharmacy, Faculty of Science, Tadulako University, Palu 94118, Indonesia.
Institute of Tropical Disease, Airlangga University, Surabaya 60115, Indonesia.
Heliyon. 2021 Apr 8;7(4):e06710. doi: 10.1016/j.heliyon.2021.e06710. eCollection 2021 Apr.
K. Schum A.D. Poulsen, and A.D. Poulsen are endemic Zingiberaceae plants from Central Sulawesi, Indonesia. This study is the first report on screening the potential antiviral activity of ethanol extracts of the leaves, pseudostems, and rhizomes parts on HIV-infected MT-4 cells and identifying chemical constituents by GC-MS. The plants were extracted by the maceration method using 96% ethanol as a solvent. The antiviral activity was measured using Viral-ToxGlo colorimetric method and using the extracts at concentrations ranging from 7.8 to 1000 μg/mL. GC-MS was used to identify the secondary metabolites of potential extracts. The results showed that ethanol extract of rhizome was the most potent antiviral activity (IC of 1.74 ± 2.46 μg/mL) and less toxic on lymphocyte (MT-4) cells (CC of 204.90 ± 106.35 μg/mL), affording the highest value of selectivity index (SI) of 117.76. rhizomes also showed promising antiviral activity with IC of 64.18 ± 2.58 μg/mL and no toxicity on MT-4 cells affording a high SI value 19.05. Preliminary GC-MS identification showed the presence of terpenoids and fatty acids as major compounds. Zerumbone, ar-turmerone, caryophyllene, and caryophyllene oxide were also detected. Chemical constituents identified by GC-MS might be responsible for the antiviral activity of extracts, suggesting further isolation and antiviral testing of the purified compounds.
K.舒姆、A.D. 波尔森和A.D. 波尔森是来自印度尼西亚中苏拉威西岛的姜科本土植物。本研究首次报道了对这些植物的叶、假茎和根茎部位的乙醇提取物在HIV感染的MT - 4细胞上的潜在抗病毒活性进行筛选，并通过气相色谱 - 质谱联用仪（GC - MS）鉴定其化学成分。采用浸渍法，以96%乙醇为溶剂对植物进行提取。使用病毒 - ToxGlo比色法，在浓度范围为7.8至1000μg/mL的提取物条件下测定抗病毒活性。利用GC - MS鉴定潜在提取物的次生代谢产物。结果表明，根茎的乙醇提取物具有最强的抗病毒活性（半数抑制浓度（IC）为1.74±2.46μg/mL），且对淋巴细胞（MT - 4）细胞毒性较小（半数细胞毒性浓度（CC）为204.90±106.35μg/mL），提供了最高的选择性指数（SI）值117.76。假茎也显示出有前景的抗病毒活性，IC为64.18±2.58μg/mL，对MT - 4细胞无毒性，提供了较高的SI值19.05。初步的GC - MS鉴定表明，萜类化合物和脂肪酸是主要化合物。还检测到了姜酮、芳姜黄酮、石竹烯和氧化石竹烯。GC - MS鉴定出的化学成分可能是提取物抗病毒活性的原因，这表明需要对纯化化合物进行进一步的分离和抗病毒测试。
