School of Materials Engineering, Purdue University, West Lafayette, Indiana 47907-2045, USA; email:
Birck Nanotechnology Center, Purdue University, West Lafayette, Indiana 47907, USA.
Annu Rev Biomed Eng. 2021 Jul 13;23:433-459. doi: 10.1146/annurev-bioeng-082020-035644. Epub 2021 Apr 19.
Since aptamers were first reported in the early 2000s, research on their use for the detection of health-relevant analytical targets has exploded. This review article provides a brief overview of the most recent developments in the field of aptamer-based biosensors for global health applications. The review provides a description of general aptasensing principles and follows up with examples of recent reports of diagnostics-related applications. These applications include detection of proteins and small molecules, circulating cancer cells, whole-cell pathogens, extracellular vesicles, and tissue diagnostics. The review also discusses the main challenges that this growing technology faces in the quest of bringing these new devices from the laboratory to the market.
自 21 世纪初首次报道适体以来，针对适体在与健康相关的分析物检测中的应用的研究呈爆炸式增长。本文简要综述了适体基生物传感器在全球健康应用领域的最新进展。本文首先介绍了适体传感的一般原理，然后列举了近期与诊断相关的应用报告实例。这些应用包括蛋白质和小分子、循环癌细胞、全细胞病原体、细胞外囊泡和组织诊断的检测。本文还讨论了该技术在将这些新设备从实验室推向市场的过程中所面临的主要挑战。
