Van Dam Nicole M, Witjes Leontien, Svatoš Aleš
Netherlands Institute of Ecology (NIOO-KNAW), PO Box 40, 6666 ZG Heteren, The Netherlands.
Max Planck Institute of Chemical Ecology, Mass Spectrometry Group, Hans-Knoll-Strasse 8, 07745 Jena, Germany.
New Phytol. 2004 Mar;161(3):801-810. doi: 10.1111/j.1469-8137.2004.00984.x.
• Interactions between shoot and root induction of glucosinolates in two crucifers, Brassica oleracea and B. nigra, were studied by applying the signalling hormones jasmonic acid (JA) and salicylic acid (SA). • JA application increased total shoot glucosinolate levels 1.5-3 times, but total root levels did not increase. Only root JA-application yielded a systemic response. In B. oleracea it mattered where JA was applied: root application increased aliphatic glucosinolates in the shoot, whereas shoot application increased indole glucosinolates. Plants treated with JA to both organs had profiles similar to shoot-treated plants. SA-application did not disturb the organ-specific response to JA. Increases in glucosinolate levels did not reduce plant biomass. • A applications reduced root glucosinolates in root-treated plants. SA root-application in B. nigra resulted in lesions on the leaves and shoot-application caused a trichome response. • lants thus respond specifically, depending on the organ that is induced and the hormone that is applied. We find a large potential for root-feeders to affect shoot-feeders. Glucosinolate induction in one organ is not constrained by induction in the other organ.
• 通过施用信号激素茉莉酸（JA）和水杨酸（SA），研究了两种十字花科植物甘蓝（Brassica oleracea）和黑芥（B. nigra）中芥子油苷的地上部和根部诱导之间的相互作用。
• 施用JA使地上部芥子油苷总水平提高了1.5至3倍，但根部总水平未增加。仅根部施用JA产生了系统性反应。在甘蓝中，JA施用于何处很重要：根部施用增加了地上部的脂肪族芥子油苷，而地上部施用增加了吲哚族芥子油苷。对两个器官都施用JA的植物的谱与仅对地上部施用JA的植物相似。施用SA并未干扰对JA的器官特异性反应。芥子油苷水平的增加并未降低植物生物量。
• SA施用降低了根部处理植物的根部芥子油苷含量。在黑芥中，根部施用SA导致叶片出现损伤，地上部施用SA引起毛状体反应。
• 因此，植物根据被诱导的器官和施用的激素做出特异性反应。我们发现根部取食者对地上部取食者有很大的影响潜力。一个器官中芥子油苷的诱导不受另一个器官中诱导的限制。
