Fink Siegried
Botanisches Institut, Auf der Morgenstelle 1, D-7400 Tuebingen, Germany.
New Phytol. 1991 Sep;119(1):33-40. doi: 10.1111/j.1469-8137.1991.tb01005.x.
The distribution of bound calcium in spruce needles is visualized by various microscopical techniques. Ca pectate occurs in the middle lamellae of cell walls and in a surprisingly great concentration in the thick cell walls of the sieve cells and transfusion parenchyma. The major fraction of the calcium, however, is bound in the form of insoluble Ca oxalate crystals, which occur extracellularly on the outside of the walls of mesophyll cells which face the intercellular spaces. Furthermore, numerous small crystals appear within the cell walls of the epidermal cells, especially in the cuticular layer. The development and distribution of these apoplastic crystals is described in detail. Some hypotheses are finally presented for possible interpretations of these unusual patterns of the crystallization of Ca oxalate outside the vacuole.
通过各种显微镜技术观察了云杉针叶中结合钙的分布情况。果胶酸钙存在于细胞壁的中层，在筛管细胞和传递薄壁组织的厚细胞壁中浓度惊人地高。然而，大部分钙以不溶性草酸钙晶体的形式结合，这些晶体在叶肉细胞面向细胞间隙的细胞壁外侧细胞外出现。此外，在表皮细胞的细胞壁内，尤其是在角质层中出现了许多小晶体。详细描述了这些质外体晶体的发育和分布。最后提出了一些假设，以解释液泡外草酸钙结晶的这些异常模式。
