State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
J Nanosci Nanotechnol. 2021 Oct 1;21(10):5036-5043. doi: 10.1166/jnn.2021.19334.
In this study, we developed a monoclonal antibody against 2,3',4,5',6-pentabromodiphenylether (BDE-121) using a synthesized hapten, and established an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA), using gold nanoparticles, to amplify the signal. The monoclonal antibody showed high specificity, with a half inhibitory concentration (IC) value of 2.78 ng/mL, towards BDE-121. The developed IC-ELISA exhibited high sensitivity and stability as well as good recovery. The intra-assay deviation is below 6.8% and the inter-assay deviations range from 6.5% to 8.7%. The assay of the actual samples was found to be consistent with those of gas chromatography/mass spectrometry (GC/MS).
在这项研究中，我们使用合成的半抗原开发了一种针对 2,3',4,5',6-五溴二苯醚（BDE-121）的单克隆抗体，并建立了一种间接竞争酶联免疫吸附测定（IC-ELISA），使用金纳米粒子来放大信号。该单克隆抗体对 BDE-121 表现出高特异性，半数抑制浓度（IC）值为 2.78ng/mL。开发的 IC-ELISA 表现出高灵敏度、稳定性和良好的回收率。内测定偏差低于 6.8%，间测定偏差范围为 6.5%至 8.7%。实际样品的测定结果与气相色谱/质谱（GC/MS）的结果一致。
