Badrulisham Aqilah Sakinah, Abu Bakar Muhammad Abdul-Latiff, Md Zain Badrul-Munir, Md-Nor Shukor, Abd Rahman Mohd-Ridwan, Mohd-Yusof Nur Syafika, Halim Madihah, Yaakop Salmah
Centre for Insect Systematics, Department of Biological Science and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
Department of Biological Science and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
Trop Life Sci Res. 2022 Mar;33(1):23-42. doi: 10.21315/tlsr2022.33.1.2. Epub 2022 Mar 31.
Microbiome studies of the parasitoid wasp, (Nixon) (Hymenoptera, Braconidae) are important because has potential as a biological control agent to suppress the pest, Walker (Lepidoptera, Psychidae). Three field populations of parasitic wasps with different Integrated Pest Management (IPM) practices to control collected from Perak state (Tapah) and Johor state (Yong Peng and Batu Pahat districts) in Peninsular Malaysia were studied. Bacterial community composition and structure were analysed using α and β diversity metrics. Proteobacteria (83.31%) and Bacteroidetes (6.80%) were the most dominant phyla, whereas unknown family from order Rhizobiales was the most abundant family found in all populations followed by Pseudomonadaceae. Family Micrococcaceae was absent in Tapah. Rhizobiales gen. sp. and sp. were abundant in all populations. Pearson's correlation analysis showed the strongest correlation between individuals of Batu Pahat and Yong Peng ( = 0.89827, < 0.05), followed by Tapah and Yong Peng with = 0.75358, < 0.05 and Batu Pahat and Tapah ( = 0.69552, < 0.05). We hypothesise that low diversity and richness in Tapah might be due to direct and indirect effect of insecticides application. This preliminary data was the first study to do inventory of the microbiomes in the gut of the .
对寄生蜂（尼克松）（膜翅目，茧蜂科）的微生物组研究很重要，因为它有潜力作为生物防治剂来抑制害虫沃克（鳞翅目，蓑蛾科）。研究了从马来西亚半岛霹雳州（塔帕）和柔佛州（永平及峇株巴辖地区）采集的、采用不同综合虫害管理（IPM）措施来防治沃克的三个寄生蜂野外种群。使用α和β多样性指标分析细菌群落组成和结构。变形菌门（83.31%）和拟杆菌门（6.80%）是最主要的门类，而根瘤菌目未知科是在所有种群中发现的最丰富的科，其次是假单胞菌科。塔帕没有微球菌科。根瘤菌目属种和种在所有种群中都很丰富。皮尔逊相关性分析表明，峇株巴辖和永平个体之间的相关性最强（= 0.89827，< 0.05），其次是塔帕和永平，= 0.75358，< 0.05以及峇株巴辖和塔帕（= 0.69552，< 0.05）。我们推测，塔帕的低多样性和丰富度可能是由于杀虫剂施用的直接和间接影响。这些初步数据是首次对该寄生蜂肠道微生物组进行清查的研究。
