[两种屏障膜和脱蛋白牛骨矿物质对拔牙窝骨再生的疗效：一项犬类微计算机断层扫描研究]
[Efficacy of two barrier membranes and deproteinized bovine bone mineral on bone regeneration in extraction sockets: A microcomputed tomographic study in dogs].
作者信息
Wang S W, You P Y, Liu Y H, Wang X Z, Tang L, Wang M
机构信息
Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.
出版信息
Beijing Da Xue Xue Bao Yi Xue Ban. 2021 Mar 3;53(2):364-370. doi: 10.19723/j.issn.1671-167X.2021.02.022.
OBJECTIVE
To evaluate the effect of two barrier membranes [multilaminated small intestinal submucosa (mSIS) and bioresorable collagen membrane (Bio-Gide)] combined with deproteinized bovine bone mineral Bio-Oss on guided bone regeneration through a canine extraction sockets model.
METHODS
The distal roots of 18 premolars of the Beagle' s bilateral maxillary and mandibular were removed, and 18 extraction sockets were obtained. They were randomly divided into 3 groups, and the following procedures were performed on the sockets: (1) filled with Bio-Oss and covered by mSIS (mSIS group), (2) filled with Bio-Oss and covered by Bio-Gide (BG group), (3) natural healing (blank control group). Micro-computed tomograph (Micro-CT) was performed 4 and 12 weeks after surgery to eva-luate the new bone regeneration in the sockets of each group.
RESULTS
The postoperative healing was uneventful in all the animals, and no complications were observed through the whole study period. Micro-CT analysis showed that the new bone fraction in the mSIS group and the BG group was significantly higher than that in the blank control group at the end of 4 weeks and 12 weeks ( < 0.05), and more new bone fraction was observed in the mSIS group than in the BG group, but the difference was not statistically significant (>0.05). The new bone fraction of coronal third part of the socket in the mSIS group and BG group at the end of 4 weeks were significantly higher than that of the middle and apical third part of each group ( < 0.05). The values of bone mineral density were similar at 4 weeks in all the groups (>0.05), but were significantly higher than that in the control group at the end of 12 weeks ( < 0.05). The bone morphometric analysis showed that the trabecular number and trabecular spacing were significantly better in the mSIS group and the BG group than in the control group at the end of 4 weeks and 12 weeks ( < 0.05), while the value in the mSIS group was slightly higher than in the BG group, but the difference was not statistically significant (>0.05). The difference in trabecular thickness between all the groups was not statistically significant (>0.05).
CONCLUSION
mSIS membrane as a barrier membrane combined with deproteinized bovine bone mineral can enhance new bone formation in canine extraction sockets, similar to Bio-Gide collagen membrane.
目的
通过犬拔牙窝模型，评估两种屏障膜[多层小肠黏膜下层（mSIS）和可吸收胶原膜（Bio-Gide）]联合脱蛋白牛骨矿物质Bio-Oss对引导性骨再生的效果。
方法
拔除18只比格犬双侧上颌和下颌前磨牙的远中根，获得18个拔牙窝。将其随机分为3组，对拔牙窝进行以下操作：（1）填充Bio-Oss并覆盖mSIS（mSIS组），（2）填充Bio-Oss并覆盖Bio-Gide（BG组），（3）自然愈合（空白对照组）。术后4周和12周进行显微计算机断层扫描（Micro-CT），以评估每组拔牙窝内的新骨再生情况。
结果
所有动物术后愈合良好，在整个研究期间未观察到并发症。Micro-CT分析显示，4周和12周结束时，mSIS组和BG组的新骨分数显著高于空白对照组（P<0.05），且mSIS组观察到的新骨分数高于BG组，但差异无统计学意义（P>0.05）。4周结束时，mSIS组和BG组拔牙窝冠方三分之一处的新骨分数显著高于每组的中部和根尖三分之一处（P<0.05）。所有组在4周时骨密度值相似（P>0.05），但在12周结束时显著高于对照组（P<0.05）。骨形态计量学分析显示，4周和12周结束时，mSIS组和BG组的骨小梁数量和骨小梁间距显著优于对照组（P<0.05），而mSIS组的值略高于BG组，但差异无统计学意义（P>0.05）。所有组之间的骨小梁厚度差异无统计学意义（P>0.05）。
结论
mSIS膜作为屏障膜联合脱蛋白牛骨矿物质可增强犬拔牙窝内的新骨形成，与Bio-Gide胶原膜相似。
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