Department of Biological Sciences and Bioengineering, Inha University, Incheon 22212, Republic of Korea.
Center for Molecular and Cell Biology, Inha University, Incheon 22212, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Apr;71(4). doi: 10.1099/ijsem.0.004771.
A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated as IMCC34836, was isolated from a freshwater stream. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IMCC34836 was most closely related to HW001 (of the family ) with 95.6 % sequence similarity and formed a robust clade with HW001. The draft genome sequence of strain IMCC34836 was 4.4 Mbp in size with 59.1 mol% DNA G+C content. Average nucleotide identity and digital DNA-DNA hybridization values between strain IMCC34836 and HW001 were 71.2 and 22.0 %, respectively, indicating that the new strain represents a novel species. The strain contained iso-C, summed feature 3 (C 6 and/or C 7) and summed feature 9 (iso-C 9 and/or C 10-methyl) as the major fatty acids and harboured phosphatidylethanolamine, two unidentified aminophospholipids and three unidentified lipids as major polar lipids. The isoprenoid quinone detected in the strain was ubiquinone-8. Based on the phylogenetic and phenotypic characteristics, strain IMCC34836 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IMCC34836 (=KACC 21755=NBRC 114416).
一株革兰氏阴性、需氧、不运动的杆状细菌，被命名为 IMCC34836，从一条淡水溪流中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 IMCC34836 与 HW001（属于 科）最为密切相关，序列相似性为 95.6%，并与 HW001 形成一个稳健的分支。菌株 IMCC34836 的基因组草图大小为 4.4 Mbp，G+C 含量为 59.1 mol%。菌株 IMCC34836 和 HW001 之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 71.2%和 22.0%，表明新菌株代表了一个新的物种。该菌株含有 iso-C、特征 3（C6 和/或 C7）和特征 9（iso-C9 和/或 C10-甲基）作为主要脂肪酸，并含有磷脂酰乙醇胺、两种未鉴定的氨基磷脂和三种未鉴定的脂质作为主要极性脂质。该菌株中检测到的异戊二烯醌是泛醌-8。基于系统发育和表型特征，菌株 IMCC34836 被认为代表了一个属的新种，建议将其命名为 sp. nov.。模式菌株为 IMCC34836（=KACC 21755=NBRC 114416）。
