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新种,分离自淡水溪流。

sp. nov., isolated from a freshwater stream.

机构信息

Department of Biological Sciences and Bioengineering, Inha University, Incheon 22212, Republic of Korea.

Center for Molecular and Cell Biology, Inha University, Incheon 22212, Republic of Korea.

出版信息

Int J Syst Evol Microbiol. 2021 Apr;71(4). doi: 10.1099/ijsem.0.004771.

Abstract

A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated as IMCC34836, was isolated from a freshwater stream. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IMCC34836 was most closely related to HW001 (of the family ) with 95.6 % sequence similarity and formed a robust clade with HW001. The draft genome sequence of strain IMCC34836 was 4.4 Mbp in size with 59.1 mol% DNA G+C content. Average nucleotide identity and digital DNA-DNA hybridization values between strain IMCC34836 and HW001 were 71.2 and 22.0 %, respectively, indicating that the new strain represents a novel species. The strain contained iso-C, summed feature 3 (C 6 and/or C 7) and summed feature 9 (iso-C 9 and/or C 10-methyl) as the major fatty acids and harboured phosphatidylethanolamine, two unidentified aminophospholipids and three unidentified lipids as major polar lipids. The isoprenoid quinone detected in the strain was ubiquinone-8. Based on the phylogenetic and phenotypic characteristics, strain IMCC34836 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IMCC34836 (=KACC 21755=NBRC 114416).

摘要

一株革兰氏阴性、需氧、不运动的杆状细菌,被命名为 IMCC34836,从一条淡水溪流中分离得到。基于 16S rRNA 基因序列的系统发育分析表明,菌株 IMCC34836 与 HW001(属于 科)最为密切相关,序列相似性为 95.6%,并与 HW001 形成一个稳健的分支。菌株 IMCC34836 的基因组草图大小为 4.4 Mbp,G+C 含量为 59.1 mol%。菌株 IMCC34836 和 HW001 之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 71.2%和 22.0%,表明新菌株代表了一个新的物种。该菌株含有 iso-C、特征 3(C6 和/或 C7)和特征 9(iso-C9 和/或 C10-甲基)作为主要脂肪酸,并含有磷脂酰乙醇胺、两种未鉴定的氨基磷脂和三种未鉴定的脂质作为主要极性脂质。该菌株中检测到的异戊二烯醌是泛醌-8。基于系统发育和表型特征,菌株 IMCC34836 被认为代表了一个属的新种,建议将其命名为 sp. nov.。模式菌株为 IMCC34836(=KACC 21755=NBRC 114416)。

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