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非霍奇金B细胞淋巴瘤中新型和复发性联合基因的检测

Detection of novel and recurrent conjoined genes in non-Hodgkin B-cell lymphoma.

作者信息

Matsumoto Yosuke, Tsukamoto Taku, Chinen Yoshiaki, Shimura Yuji, Sasaki Nana, Nagoshi Hisao, Sato Ryuichi, Adachi Hiroko, Nakano Masakazu, Horiike Shigeo, Kuroda Junya, Taki Tomohiko, Tashiro Kei, Taniwaki Masafumi

机构信息

Department of Hematology, Japanese Red Cross Kyoto Daiichi Hospital, Kyoto, Japan.

Division of Hematology and Oncology, Kyoto Prefectural University of Medicine, Kyoto, Japan.

出版信息

J Clin Exp Hematop. 2021 Jun 5;61(2):71-77. doi: 10.3960/jslrt.20033. Epub 2021 Apr 20.

DOI:10.3960/jslrt.20033
PMID:33883344
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8265495/
Abstract

For this study, we investigated comprehensive expression of conjoined genes (CGs) in non-Hodgkin B-cell lymphoma (B-NHL) cell line KPUM-UH1 by using paired-end RNA sequencing. Furthermore, we analyzed the expression of these transcripts in an additional 21 cell lines, 37 primary samples of various malignancies and peripheral blood mononuclear cells of four normal individuals. Seventeen CGs were detected in KPUM-UH1: CTBS-GNG5, SRP9-EPHX1, RMND5A-ANAPC, OTX1-EHBP1, ATF2-CHN1, PRKAA1-TTC33, LARP1-MRPL22, LOC105379697-BAK1, TIAM2-SCAF8, SPAG1-VPS13B, WBP1L-CNNM2, NARS2-GAB2, CTSC-RAB38, VAMP1-CD27-AS1, LRRC37A2-NSF, UBA2-WTIP and ZNF600-ZNF611. To our knowledge, 10 of these genes have not been previously reported. The various characteristics of the CGs included in- and out-of-frame fusions, chimeras involving non-coding RNA and transcript variants. A finding of note was that LARP1-MRPL2 was characterized as in-frame fusion and was recurrently expressed in B-NHL samples. In this study, variety of CGs was expressed both in malignant and normal cells, some of which might be specific to lymphoma.

摘要

在本研究中，我们通过使用双末端RNA测序技术，研究了非霍奇金B细胞淋巴瘤（B-NHL）细胞系KPUM-UH1中融合基因（CGs）的全面表达情况。此外，我们还分析了这些转录本在另外21个细胞系、37个各种恶性肿瘤的原发性样本以及4名正常个体的外周血单个核细胞中的表达情况。在KPUM-UH1中检测到17个融合基因：CTBS-GNG5、SRP9-EPHX1、RMND5A-ANAPC、OTX1-EHBP1、ATF2-CHN1、PRKAA1-TTC33、LARP1-MRPL22、LOC105379697-BAK1、TIAM2-SCAF8、SPAG1-VPS13B、WBP1L-CNNM2、NARS2-GAB2、CTSC-RAB38、VAMP1-CD27-AS1、LRRC37A2-NSF、UBA2-WTIP和ZNF600-ZNF611。据我们所知，其中10个基因此前尚未见报道。这些融合基因的各种特征包括框内和框外融合、涉及非编码RNA的嵌合体以及转录变体。值得注意的是，LARP1-MRPL2被鉴定为框内融合，并且在B-NHL样本中反复表达。在本研究中，多种融合基因在恶性和正常细胞中均有表达，其中一些可能是淋巴瘤特有的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cda/8265495/2babcea083c5/jslrt-61-71-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cda/8265495/bc5f93538251/jslrt-61-71-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cda/8265495/fbc1740845e8/jslrt-61-71-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cda/8265495/2babcea083c5/jslrt-61-71-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cda/8265495/bc5f93538251/jslrt-61-71-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cda/8265495/fbc1740845e8/jslrt-61-71-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cda/8265495/2babcea083c5/jslrt-61-71-g003.jpg

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