Yang Chen, Zhu Suting, Sun Chaoling, Ding Lijian, Jung Dawoon, He Shan, Wang Ning, Zhang Weiyan
Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, PR China.
Institute of Drug Discovery Technology, Ningbo University, Ningbo 315211, PR China.
Int J Syst Evol Microbiol. 2022 Apr;72(4). doi: 10.1099/ijsem.0.005325.
A Gram-stain-negative, rod-shaped, motile and aerobic marine bacterium, designated strain NBU2595, was isolated from marine sediment sampled on Meishan Island, located in the East China Sea. Strain NBU2595 grew at 10-40 °C (optimum, 37 °C), at NaCl concentration of 0-10.0 % (w/v; optimum, 0.5 %) and at pH 6.0-8.0 (optimum, pH 7.0). Catalase and oxidase activities and HS production were positive. Methyl red reaction and hydrolysis of casein, starch and Tweens 20, 40, 60 and 80 were negative. The major cellular fatty acids were summed feature 8 (C 7 and/or C 6), C 2-OH and C 3-OH. The sole respiratory quinone was ubiquinone 10. The major polar lipids were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylmonomethylethanolamine. Comparative analysis of 16S rRNA gene sequences showed highest similarity to CL-UU02 (97.9%), and low similarities (<92.9 %) to other species. Phylogenetic analyses indicated that strain NBU2595 clustered with the genus and was closely related to CL-UU02. The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU2595 and the related species of the genus were well below the thresholds for prokaryotic species delineation. The DNA G+C content was 66.5 mol%. Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU2595 should be placed in the genus as representing a novel species, for which the name sp. nov. is proposed. The type strain is NBU2595 (=MCCC 1K04773=KCTC 82223).
从中国东海梅山岛采集的海洋沉积物中分离出一株革兰氏阴性、杆状、具运动性的需氧海洋细菌，命名为NBU2595菌株。NBU2595菌株在10-40°C（最适温度为37°C）、NaCl浓度为0-10.0%（w/v；最适浓度为0.5%）以及pH值为6.0-8.0（最适pH值为7.0）的条件下生长。过氧化氢酶和氧化酶活性以及H₂S产生均为阳性。甲基红反应以及酪蛋白、淀粉和吐温20、40、60和80的水解反应均为阴性。主要细胞脂肪酸为总和特征8（C₁₇:₇和/或C₁₆:₆）、C₁₉:₀ 2-OH和C₁₉:₀ 3-OH。唯一的呼吸醌为泛醌10。主要极性脂为磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰单甲基乙醇胺。16S rRNA基因序列的比较分析显示与CL-UU02的相似性最高（97.9%），与其他物种的相似性较低（<92.9%）。系统发育分析表明NBU2595菌株与该属聚类且与CL-UU02密切相关。NBU2595菌株与该属相关物种之间的平均核苷酸同一性和数字DNA-DNA杂交值远低于原核生物物种界定的阈值。DNA G+C含量为66.5 mol%。基于其表型、化学分类和基因型数据，NBU2595菌株应归入该属，代表一个新物种，为此提出新物种名称[具体新物种名称未给出]。模式菌株为NBU2595（=MCCC 1K04773=KCTC 82223）。
