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猪下丘脑和发情周期及早期妊娠血浆趋化素及其受体的表达。

Expression of Chemerin and Its Receptors in the Porcine Hypothalamus and Plasma Chemerin Levels during the Oestrous Cycle and Early Pregnancy.

机构信息

Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, Poland.

出版信息

Int J Mol Sci. 2019 Aug 9;20(16):3887. doi: 10.3390/ijms20163887.

DOI:10.3390/ijms20163887
PMID:31404950
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6719988/
Abstract

Chemerin (CHEM) may act as an important link integrating energy homeostasis and reproductive functions of females, and its actions are mediated by three receptors: chemokine-like receptor 1 (CMKLR1), G protein-coupled receptor 1 (GPR1), and C-C motif chemokine receptor-like 2 (CCRL2). The aim of the current study was to compare the expression of CHEM and its receptor (CHEM system) mRNAs (quantitative real-time PCR) and proteins (Western blotting and fluorescent immunohistochemistry) in the selected areas of the porcine hypothalamus responsible for gonadotropin-releasing hormone production and secretion: the mediobasal hypothalamus, preoptic area and stalk median eminence during the oestrous cycle and early pregnancy. Moreover, plasma CHEM concentrations were determined using ELISA. The expression of CHEM system has been demonstrated in the porcine hypothalamus throughout the luteal phase and follicular phase of the oestrous cycle, and during early pregnancy from days 10 to 28. Plasma CHEM levels and concentrations of transcripts and proteins of CHEM system components in the hypothalamus fluctuated throughout pregnancy and the oestrous cycle. Our study was the first experiment to demonstrate the presence of CHEM system mRNAs and proteins in the porcine hypothalamus and the correlations between the expression levels and physiological hormonal milieu related to the oestrous cycle and early pregnancy.

摘要

趋化素 (CHEM) 可能作为连接雌性动物能量稳态和生殖功能的重要纽带，其作用由三个受体介导：趋化因子样受体 1 (CMKLR1)、G 蛋白偶联受体 1 (GPR1) 和 C-C 基序趋化因子受体样 2 (CCRL2)。本研究旨在比较发情周期和早期妊娠期间猪下丘脑与促性腺激素释放激素产生和分泌相关的选定区域（中脑基底部、视前区和柄部中间隆起）中 CHEM 和其受体（CHEM 系统）mRNA（实时定量 PCR）和蛋白（Western 印迹和荧光免疫组织化学）的表达。此外，还使用 ELISA 测定了血浆 CHEM 浓度。在发情周期的黄体期和卵泡期以及早期妊娠的第 10 至 28 天，猪下丘脑均有 CHEM 系统的表达。血浆 CHEM 水平以及下丘脑 CHEM 系统各组成部分的转录物和蛋白浓度在整个妊娠和发情周期中波动。本研究首次证明了 CHEM 系统 mRNA 和蛋白存在于猪的下丘脑，并证明了表达水平与发情周期和早期妊娠相关的生理激素环境之间存在相关性。

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