Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
G3 (Bethesda). 2021 Jul 14;11(7). doi: 10.1093/g3journal/jkab137.
We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50 of 886 Kb, including a circular mitochondrial sequence of 28 Kb. Using direct RNA nanopore sequencing and Illumina cDNA sequencing, we constructed an annotation of our new assembly, supplemented by lifting over genes from Saccharomyces cerevisiae and Candida glabrata.
我们呈现了致病性酵母念珠菌属的高度连续基因组和转录组。我们使用牛津纳米孔技术和 Illumina 平台对该物种的 DNA 和 RNA 进行了测序。我们将基因组组装成一个 11.8Mb 的草图，由 16 个 contigs 组成，N50 为 886 Kb，包括一个 28 Kb 的圆形线粒体序列。使用直接 RNA 纳米孔测序和 Illumina cDNA 测序，我们构建了我们的新组装注释，辅之以从酿酒酵母和光滑念珠菌中转移的基因。
