Division of Oral and Maxillofacial Surgery, Department of Oral Medicine and Surgery, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan; Division of Oral and Maxillofacial Surgery, Department of Medicine of Sensory and Motor Organs, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan.
Division of Oral Pathology, Department of Oral Medicine and Surgery, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan.
Transfus Apher Sci. 2021 Aug;60(4):103144. doi: 10.1016/j.transci.2021.103144. Epub 2021 Apr 20.
Fibrin glue from autologous plasma may prevent viral infection and allergic reaction. Moreover, this biomaterial contains growth factors such as TGF-β and VEGF that promote reconstruction of the mucous membrane by stimulating fibroblast proliferation and angiogenesis. Thus, autologous fibrin glue is predicted to improve healing better than commercial fibrin glue. Here, we evaluated the effects of autologous fibrin glue on the crucial early phase of wound healing. Epithelial defects were introduced in rats and covered with polyglycolic acid (PGA) sheets with or without commercial or autologous fibrin glue. Wound healing was assessed for six weeks by histology and immunohistochemistry. Our results demonstrate that wounds covered with PGA sheets and autologous fibrin glue achieved efficient wound healing without complications such as local infection or incomplete healing. The rate of recovery of the regenerating epithelium in this group was superior to that in wounds covered with PGA sheets and commercial fibrin glue. Immunohistochemistry of laminin, cytokeratin, and VEGF confirmed fine and rapid epithelial neogenesis. Collectively, our results indicate that covering surgical wounds with autologous fibrin glue promotes wound healing and epithelialization, improves safety, and reduces the risks of viral infection and allergic reaction associated with conventional techniques.
自体血浆纤维蛋白胶可预防病毒感染和过敏反应。此外，这种生物材料还含有 TGF-β和 VEGF 等生长因子，通过刺激成纤维细胞增殖和血管生成，促进黏膜重建。因此，自体纤维蛋白胶有望比商业纤维蛋白胶更好地促进愈合。在这里，我们评估了自体纤维蛋白胶对创伤愈合关键早期阶段的影响。在大鼠中引入上皮缺损，并使用聚乙二醇酸（PGA）片覆盖，有或没有商业或自体纤维蛋白胶。通过组织学和免疫组织化学评估了六周的伤口愈合情况。我们的结果表明，用 PGA 片和自体纤维蛋白胶覆盖的伤口实现了有效的愈合，没有局部感染或愈合不全等并发症。该组再生上皮的恢复速度优于用 PGA 片和商业纤维蛋白胶覆盖的伤口。层粘连蛋白、细胞角蛋白和 VEGF 的免疫组织化学证实了精细和快速的上皮新生。总的来说，我们的结果表明，用自体纤维蛋白胶覆盖手术伤口可促进伤口愈合和上皮化，提高安全性，并降低与传统技术相关的病毒感染和过敏反应的风险。
