Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China; University of the Chinese Academy of Sciences, Beijing, PR China; Xiamen Key Laboratory of Physical Environment, Xiamen, 361021, China.
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China; Xiamen Key Laboratory of Physical Environment, Xiamen, 361021, China; Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences, Shanghai, 200031, China.
Environ Pollut. 2021 Aug 15;283:117087. doi: 10.1016/j.envpol.2021.117087. Epub 2021 Apr 5.
With the rapid development of science and technology, 5G technology will be widely used, and biosafety concerns about the effects of 5G radiofrequency radiation on health have been raised. Drosophila melanogaster was selected as the model organism for our study, in which a 3.5 GHz radiofrequency radiation (RF-EMR) environment was simulated at intensities of 0.1 W/m, 1 W/m, and 10 W/m. The activity of parent male and offspring (F1) male flies was measured using a Drosophila activity monitoring system under short-term and long-term 3.5 GHz RF-EMR exposure. Core genes associated with heat stress, the circadian clock and neurotransmitters were detected by QRT-PCR technology, and the contents of GABA and glutamate were detected by UPLC-MS. The results show that short-term RF-EMR exposure increased the activity level and reduced the sleep duration while long-term RF-EMR exposure reduced the activity level and increased the sleep duration of F1 male flies. Under long-term RF-EMR, the expression of heat stress response-related hsp22, hsp26 and hsp70 genes was increased, the expression of circadian clock-related per, cyc, clk, cry, and tim genes was altered, the content of GABA and glutamate was reduced, and the expression levels of synthesis, transport and receptor genes were altered. In conclusion, long-term RF-EMR exposure enhances the heat stress response of offspring flies and then affects the expression of circadian clock and neurotransmitter genes, which leads to decreased activity, prolonged sleep duration, and improved sleep quality.
随着科学技术的飞速发展，5G 技术将得到广泛应用，人们对 5G 射频辐射对健康影响的生物安全性提出了担忧。本研究选择黑腹果蝇作为模式生物，在 0.1、1 和 10 W/m 三种强度的 3.5GHz 射频电磁辐射（RF-EMR）环境下模拟短期和长期暴露。利用果蝇活动监测系统测量短期和长期 3.5GHz RF-EMR 暴露下亲代雄蝇和子代（F1）雄蝇的活动。采用 QRT-PCR 技术检测与热应激、生物钟和神经递质相关的核心基因，采用 UPLC-MS 检测 GABA 和谷氨酸的含量。结果表明，短期 RF-EMR 暴露增加了 F1 雄蝇的活动水平，减少了睡眠时间，而长期 RF-EMR 暴露降低了 F1 雄蝇的活动水平，增加了睡眠时间。在长期 RF-EMR 下，热应激反应相关基因 hsp22、hsp26 和 hsp70 的表达增加，生物钟相关基因 per、cyc、clk、cry 和 tim 的表达发生改变，GABA 和谷氨酸的含量降低，合成、转运和受体基因的表达水平也发生了改变。综上所述，长期 RF-EMR 暴露增强了子代果蝇的热应激反应，进而影响生物钟和神经递质基因的表达，导致活动减少、睡眠时间延长和睡眠质量提高。
