Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Professor García González 2, 41012, Seville, Spain.
IHU-Méditerranée Infection, Marseille, France; Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France.
Comp Immunol Microbiol Infect Dis. 2021 Jun;76:101648. doi: 10.1016/j.cimid.2021.101648. Epub 2021 Apr 6.
In this study, we assessed the presence of vector-borne microorganisms in different species of fleas collected from different hosts in diverse areas of South-Western Europe by molecular methods. A total of 319 fleas belonging to eight different species was tested for the presence of eight microorganisms. Wolbachia spp. endosymbionts were detected in Ctenocephalides felis, Pulex irritans, Archaeopsylla erinacei and Ctenophthalmus baeticus boisseauorum specimens. Rickettsia felis, an emerging pathogen, was detected in C. felis, A. erinacei and Ct. b. boisseauorum. Rickettsia typhi, the agent of murine typhus was detected for the first time in A. erinacei and Mycobacterium spp. were detected for the first time in fleas (C. felis, P. irritans and A. erinacei). Lastly, five different species of Bartonella were detected in fleas' DNA in this study, including a possible new bacterium belonging to this genus. With this study, we updated the knowledge of the flea-borne bacteria present in the South-West of Europe reinforcing the idea about the necessity to expand and increase the current knowledge on flea-borne pathogens.
在这项研究中，我们通过分子方法评估了来自欧洲西南部不同地区不同宿主的不同跳蚤物种中存在的媒介传播微生物。共检测了 319 只属于八个不同物种的跳蚤，以检测八种微生物的存在。在 Ctenocephalides felis、Pulex irritans、Archaeopsylla erinacei 和 Ctenophthalmus baeticus boisseauorum 标本中检测到沃尔巴克氏体属内共生菌。在 C. felis、A. erinacei 和 Ct. b. boisseauorum 中检测到新兴病原体猫立克次体。在 A. erinacei 中首次检测到鼠伤寒沙门氏菌，在跳蚤（C. felis、P. irritans 和 A. erinacei）中首次检测到分枝杆菌属。最后，本研究在跳蚤的 DNA 中检测到五种不同的巴尔通体，包括该属的一种可能的新细菌。通过这项研究，我们更新了欧洲西南部存在的跳蚤传播细菌的知识，强化了扩大和增加当前对跳蚤传播病原体的认识的必要性。
