Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 139-774, Korea.
Department of Biological Science, Sangji University, Sangjidae-gil 83, Wonju-si, Gangwon-do, Republic of Korea.
Arch Microbiol. 2021 Aug;203(6):3435-3442. doi: 10.1007/s00203-021-02293-8. Epub 2021 Apr 24.
A Gram-negative, aerobic, flagellated, rod-shaped, and pink-pigmented bacterium, strain 17Sr1-43, was isolated from a soil sample collected in Nowongu, Seoul, Korea. The isolate could grow at 18-37 °C (optimum, 28-30 °C), pH 6.0-8.0 (optimum, pH 7.0) and in the presence of 0-1.0% (w/v) NaCl (optimum, 0%) with aeration. The major cellular fatty acids were summed feature 8 (C ω7c and/or C ω6c) and summed feature 2 (iso-C I and/or C 3-OH). The predominant respiratory quinone was Q-10 and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phospholipid, and diphosphatidylglycerol. The G + C content of genomic DNA was 69.1 mol%. Strain 17Sr1-43 was closely related to Methylobacterium gregans KACC 14808 (98.4% 16S rRNA gene sequence similarity), Methylobacterium hispanicum KACC 11432 (97.9%), and Methylobacterium phyllosphaerae CBMB27 (96.1%). The complete genome of strain 17Sr1-43 contains essential genes related to DNA repair processes including bacterial RecBCD dependent pathway and UmuCD system. Based on the phenotypic, genotypic, and chemotaxonomic characteristics, strain 17Sr1-43 represents a novel species in the genus Methylobacterium, for which the name Methylobacterium radiodurans sp. nov. is proposed. The type strain is strain 17Sr1-43 (= KCTC 52906 = NBRC 112875).
一株革兰氏阴性、需氧、鞭毛、杆状、粉红色着色的细菌，菌株 17Sr1-43，从韩国首尔 Nowongu 采集的土壤样本中分离得到。该分离株可在 18-37°C（最佳温度 28-30°C）、pH 值 6.0-8.0（最佳 pH 值 7.0）和有氧条件下在 0-1.0%（w/v）NaCl（最佳 0%）中生长。主要细胞脂肪酸为总和特征 8（C ω7c 和/或 C ω6c）和总和特征 2（iso-C I 和/或 C 3-OH）。主要呼吸醌为 Q-10，主要极性脂为磷脂酰乙醇胺、磷脂酰甘油、磷脂和双磷脂酰甘油。基因组 DNA 的 G+C 含量为 69.1mol%。菌株 17Sr1-43 与 Methylobacterium gregans KACC 14808（98.4% 16S rRNA 基因序列相似性）、Methylobacterium hispanicum KACC 11432（97.9%）和 Methylobacterium phyllosphaerae CBMB27（96.1%）密切相关。菌株 17Sr1-43 的全基因组包含与 DNA 修复过程相关的必需基因，包括细菌 RecBCD 依赖性途径和 UmuCD 系统。基于表型、基因型和化学分类学特征，菌株 17Sr1-43 代表甲基杆菌属中的一个新种，建议命名为甲基杆菌放射性抗性菌 sp. nov.。模式菌株为 17Sr1-43 菌株（=KCTC 52906= NBRC 112875）。
