Department of Life Science, Dongguk University-Seoul, Goyang, 10326, South Korea.
Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 01797, South Korea.
Antonie Van Leeuwenhoek. 2020 Feb;113(2):211-220. doi: 10.1007/s10482-019-01331-2. Epub 2019 Sep 20.
A gamma radiation-resistant, Gram-stain negative, oxidase and catalase positive, aerobic, flagellated, rod-shaped, methylotrophic and pink-pigmented bacterial strain designated 17SD2-17 was isolated from gamma-ray-irradiated soil collected in Korea. The 16S rRNA gene sequence analysis showed that strain 17SD2-17 is phylogenetically related to Methylobacterium organophilum DSM 760 (97.6%), Methylobacterium oxalidis 35a (97.4%) and Methylobacterium soli YIM 48816 (97.0%). The G+C content calculated based on the draft genome sequence is 68.7 mol%. The DNA-DNA hybridisation between 17SD2-17 and its close relatives was found to be less than 40%. The predominant fatty acid was identified as summed feature 8 (Cω7c and/or Cω6c) and the major respiratory quinone as Q-10. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. On the basis of the data from phenotypic tests and genotypic differences between strain 17SD2-17 and its close phylogenetic relatives, strain 17SD2-17 is concluded to represent a new species belonging to the genus Methylobacterium, for which the name Methylobacterium durans sp. nov. (= KCTC 52908 = NBRC 112876) is proposed.
一株耐伽马射线、革兰氏阴性、氧化酶和过氧化氢酶阳性、需氧、鞭毛、杆状、甲基营养型、粉红色着色的细菌菌株 17SD2-17 从韩国采集的伽马射线辐照土壤中分离得到。16S rRNA 基因序列分析表明，菌株 17SD2-17 在系统发育上与甲基杆菌属有机磷 DSM 760（97.6%）、甲基杆菌属氧化草酸 35a（97.4%）和甲基杆菌属 soli YIM 48816（97.0%）密切相关。基于基因组草图计算的 G+C 含量为 68.7 mol%。17SD2-17 与其近亲之间的 DNA-DNA 杂交率低于 40%。主要脂肪酸被鉴定为总和特征 8（Cω7c 和/或 Cω6c），主要呼吸醌为 Q-10。主要极性脂类为双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。基于表型试验和菌株 17SD2-17 与其近亲之间的基因型差异的数据，菌株 17SD2-17 被认为代表一个新的甲基杆菌属物种，建议将其命名为 Methylobacterium durans sp. nov.（=KCTC 52908= NBRC 112876）。
