Lachetta Mario, Sandmeyer Hauke, Sandmeyer Alice, Esch Jan Schulte Am, Huser Thomas, Müller Marcel
Biomolecular Photonics, Faculty of Physics, Bielefeld University, Universitätsstraße 25, 33501 Bielefeld, Germany.
Department of General and Visceral Surgery, Evangelisches Klinikum Bethel GmbH, University Hospital OWL of Bielefeld University, Campus Bielefeld-Bethel, Bielefeld, Germany.
Philos Trans A Math Phys Eng Sci. 2021 Jun 14;379(2199):20200147. doi: 10.1098/rsta.2020.0147. Epub 2021 Apr 26.
Digital micromirror devices (DMDs) are spatial light modulators that employ the electro-mechanical movement of miniaturized mirrors to steer and thus modulate the light reflected off a mirror array. Their wide availability, low cost and high speed make them a popular choice both in consumer electronics such as video projectors, and scientific applications such as microscopy. High-end fluorescence microscopy systems typically employ laser light sources, which by their nature provide coherent excitation light. In super-resolution microscopy applications that use light modulation, most notably structured illumination microscopy (SIM), the coherent nature of the excitation light becomes a requirement to achieve optimal interference pattern contrast. The universal combination of DMDs and coherent light sources, especially when working with multiple different wavelengths, is unfortunately not straight forward. The substructure of the tilted micromirror array gives rise to a which has to be understood and which must be taken into account when designing a DMD-based illumination system. Here, we present a set of simulation frameworks that explore the use of DMDs in conjunction with coherent light sources, motivated by their application in SIM, but which are generalizable to other light patterning applications. This framework provides all the tools to explore and compute DMD-based diffraction effects and to simulate possible system alignment configurations computationally, which simplifies the system design process and provides guidance for setting up DMD-based microscopes. This article is part of the Theo Murphy meeting 'Super-resolution structured illumination microscopy (part 1)'.
数字微镜器件（DMD）是一种空间光调制器，它利用小型化镜子的机电运动来引导并进而调制从镜子阵列反射的光。其广泛的可用性、低成本和高速度使其在消费电子产品（如视频投影仪）以及科学应用（如显微镜学）中都成为一种受欢迎的选择。高端荧光显微镜系统通常采用激光光源，从本质上讲，激光光源可提供相干激发光。在使用光调制的超分辨率显微镜应用中，最显著的是结构照明显微镜（SIM），激发光的相干特性成为实现最佳干涉图案对比度的必要条件。不幸的是，DMD与相干光源的通用组合，尤其是在使用多种不同波长时，并非易事。倾斜微镜阵列的子结构会产生一种 ，在设计基于DMD的照明系统时必须理解并加以考虑。在此，我们提出了一组模拟框架，受其在SIM中的应用启发，探索DMD与相干光源结合的使用情况，但这些框架可推广到其他光图案化应用。该框架提供了所有工具来探索和计算基于DMD的衍射效应，并通过计算模拟可能的系统对准配置，这简化了系统设计过程，并为设置基于DMD的显微镜提供了指导。本文是西奥·墨菲会议“超分辨率结构照明显微镜（第1部分）”的一部分。 （注：原文中“which has to be understood and which must be taken into account when designing a DMD-based illumination system.”之间的“a”未给出完整内容，翻译时保留原文格式。）
