Wang Qingzhuo, Zhang Jiao, Al Makishah Naief H, Sun Xiaoman, Wen Zhiqiang, Jiang Yu, Yang Sheng
School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, China.
Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.
Front Microbiol. 2021 Apr 7;12:654058. doi: 10.3389/fmicb.2021.654058. eCollection 2021.
has been considered a promising synthetic biological platform for biomanufacturing and bioremediation. However, there are still some challenges in genetic manipulation of . Recently, more and more genetic parts or elements (replicons, promoters, reporter genes, and selectable markers) have been mined, characterized, and applied. In addition, continuous improvement of classic molecular genetic manipulation techniques, such as allelic exchange via single/double-crossover, nuclease-mediated site-specific recombination, RecT-mediated single-chain recombination, actinophages integrase-mediated integration, and transposition mutation, has accelerated the molecular study of More importantly, emerging gene editing tools based on the CRISPR/Cas system is revolutionarily rewriting the pattern of genetic manipulation technology development for , which made gene reprogramming, such as insertion, deletion, replacement, and point mutation, much more efficient and simpler. This review summarized the recent progress in molecular genetic manipulation technology development of and discussed the bottlenecks and perspectives for future research of as a distinctive microbial chassis.
已被认为是生物制造和生物修复领域一个很有前景的合成生物学平台。然而，在对其进行基因操作方面仍存在一些挑战。最近，越来越多的基因元件（复制子、启动子、报告基因和选择标记）被挖掘、表征和应用。此外，经典分子遗传操作技术的不断改进，如通过单/双交换进行等位基因交换、核酸酶介导的位点特异性重组、RecT介导的单链重组、肌动噬菌体整合酶介导的整合以及转座突变，加速了对其的分子研究。更重要的是，基于CRISPR/Cas系统的新兴基因编辑工具正在革命性地改写其基因操作技术发展模式，这使得基因重编程，如插入、缺失、替换和点突变，变得更加高效和简单。本综述总结了其分子遗传操作技术发展的最新进展，并讨论了作为独特微生物底盘的未来研究的瓶颈和前景。
