Jeng Ming-Luen, Chen Ming-Yu, Wu Li-Wei
Department of Biology, National Museum of Natural Science, Taichung, Taiwan, ROC.
Department of Life Science, Tunghai University, Taichung, Taiwan, ROC.
Mitochondrial DNA B Resour. 2021 Apr 5;6(4):1341-1343. doi: 10.1080/23802359.2021.1909443.
Museum specimens are collected for education, exhibition, and various multiple scientific purposes. However, millions of specimens remain in their collection boxes for years without being analyzed. Historical specimens have been known to contain low-quality DNA; hence, it is difficult to utilize their sequence information in phylogenetic studies. However, recent advances in high-throughput sequencing (HTS) make these collections amenable to phylogenomic studies. In this study, two historical specimens ( Linnaeus, 1767, and Linnaeus, 1771) were sampled and DNA extracted for HTS the Miseq platform. Two complete mitogenomes were assembled, even though the DNA quality of those specimens was highly fragmented, below 250 bp in length. The 37 genes of 60 mitogenomes were aligned and used for inferring the phylogenetic relationships of Papilioninae. These two newly sequenced mitogenomes are correctly grouped in the genus , and this result indicates that historical specimens show great potential for phylogenetic studies with HTS technology.
博物馆标本的采集是为了教育、展览以及各种多样的科学目的。然而，数以百万计的标本多年来一直存放在收藏盒中未被分析。已知历史标本含有低质量的DNA；因此，在系统发育研究中难以利用它们的序列信息。然而，高通量测序（HTS）的最新进展使这些标本集适合进行系统基因组学研究。在本研究中，采集了两个历史标本（林奈，1767年，和林奈，1771年），并提取DNA用于在Miseq平台上进行高通量测序。尽管这些标本的DNA质量高度碎片化，长度低于250bp，但仍组装出了两个完整的线粒体基因组。对60个线粒体基因组的37个基因进行了比对，并用于推断凤蝶亚科的系统发育关系。这两个新测序的线粒体基因组被正确地归入该属，这一结果表明历史标本在利用高通量测序技术进行系统发育研究方面具有巨大潜力。
