Zhang Xin, Li Ying, Ma Zhijie, He Dan, Li Haiyan
School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, China.
Chemical and Environmental Engineering, School of Engineering, RMIT University, 124 La Trobe St, Melbourne, VIC, 3000, Australia.
Bioact Mater. 2021 Apr 6;6(11):3692-3704. doi: 10.1016/j.bioactmat.2021.03.038. eCollection 2021 Nov.
More and more studies have recognized that the nanosized pores of hydrogels are too small for cells to normally grow and newly formed tissue to infiltrate, which impedes tissue regeneration. Recently, hydrogels with macropores and/or controlled degradation attract more and more attention for solving this problem. Sodium alginate/Bioglass (SA/BG) hydrogel, which has been reported to be an injectable and bioactive hydrogel, is also limited to be used as tissue engineering scaffolds due to its nanosized pores. Therefore, in this study, degradation of SA/BG hydrogel was modulated by grafting deferoxamine (DFO) to SA. The functionalized grafted DFO-SA (G-DFO-SA) was used to form G-DFO-SA/BG injectable hydrogel. In vitro degradation experiments proved that, compared to SA/BG hydrogel, G-DFO-SA/BG hydrogel had a faster mass loss and structural disintegration. When the hydrogels were implanted subcutaneously, G-DFO-SA/BG hydrogel possessed a faster degradation and better tissue infiltration as compared to SA/BG hydrogel. In addition, in a rat full-thickness skin defect model, wound healing studies showed that, G-DFO-SA/BG hydrogel significantly accelerated wound healing process by inducing more blood vessels formation. Therefore, G-DFO-SA/BG hydrogel can promote tissue infiltration and stimulate angiogenesis formation, which suggesting a promising application potential in tissue regeneration.
越来越多的研究认识到，水凝胶的纳米级孔隙对于细胞正常生长和新形成组织的浸润来说太小，这阻碍了组织再生。最近，具有大孔和/或可控降解性的水凝胶在解决这一问题方面越来越受到关注。海藻酸钠/生物玻璃（SA/BG）水凝胶据报道是一种可注射的生物活性水凝胶，但由于其纳米级孔隙，在用作组织工程支架方面也受到限制。因此，在本研究中，通过将去铁胺（DFO）接枝到SA上来调节SA/BG水凝胶的降解。功能化的接枝DFO-SA（G-DFO-SA）用于形成G-DFO-SA/BG可注射水凝胶。体外降解实验证明，与SA/BG水凝胶相比，G-DFO-SA/BG水凝胶具有更快的质量损失和结构崩解。当将水凝胶皮下植入时，与SA/BG水凝胶相比，G-DFO-SA/BG水凝胶具有更快的降解速度和更好的组织浸润性。此外，在大鼠全层皮肤缺损模型中，伤口愈合研究表明，G-DFO-SA/BG水凝胶通过诱导更多血管形成显著加速了伤口愈合过程。因此，G-DFO-SA/BG水凝胶可促进组织浸润并刺激血管生成，这表明其在组织再生中具有广阔的应用潜力。
