Chen Chao, Gopinath Subash C B, Anbu Periasamy
Department of Intensive Care Units, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Henan University People's Hospital, Zhengzhou, 450000, Henan, China.
Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000 Kangar, Perlis, Malaysia.
Nanoscale Res Lett. 2021 Apr 26;16(1):68. doi: 10.1186/s11671-021-03527-w.
Sepsis is an extreme condition involving a physical response to severe microbial infection and causes fatal and life-threatening issues. Sepsis generates during the chemicals release with the immune system into the bloodstream for fighting against an infection, which causes the inflammation and leads to the medical emergency. A complexed longitudinal zeolite and iron oxide nanocomposite was extracted from coal mine fly ash and utilized to improve the surface characteristics of the capacitance biosensor to identify sepsis attacks. Anti-interleukin-3 (anti-IL-3) antibody was attached to the zeolite- and iron oxide-complexed capacitance electrode surface through an amine linker to interact with the sepsis biomarker IL-3. The morphological and chemical components of the nanocomplex were investigated by FESEM, FETEM, and EDX analyses. At approximately 30 nm, the longitudinal zeolite and iron oxide nanocomposite aided in attaining the limit of IL-3 detection of 3 pg/mL on the linear curve, with a regression coefficient (R) of 0.9673 [y = 1.638x - 1.1847]. A lower detection limit was achieved in the dose-dependent range (3-100 pg/mL) due to the higher amount of antibody immobilization on the sensing surface due to the nanomaterials and the improved surface current. Furthermore, control experiments with relevant biomolecules did not show capacitance changes, and spiked IL-3 in human serum increased capacitance, indicating the specific and selective detection of IL-3. This study identifies and quantifies IL-3 via potentially useful methods and helps in diagnosing sepsis attack.
脓毒症是一种极端情况，涉及对严重微生物感染的身体反应，并会引发致命和危及生命的问题。脓毒症在免疫系统将化学物质释放到血液中以对抗感染的过程中产生，这会引发炎症并导致医疗急症。一种复合纵向沸石和氧化铁纳米复合材料从煤矿粉煤灰中提取出来，并用于改善电容生物传感器的表面特性，以识别脓毒症发作。抗白细胞介素-3（抗IL-3）抗体通过胺连接体附着在沸石和氧化铁复合的电容电极表面，与脓毒症生物标志物IL-3相互作用。通过场发射扫描电子显微镜（FESEM）、场发射透射电子显微镜（FETEM）和能谱分析（EDX）对纳米复合材料的形态和化学成分进行了研究。纵向沸石和氧化铁纳米复合材料在约30纳米时，有助于在直线曲线上达到IL-3的检测限为3皮克/毫升，回归系数（R）为0.9673 [y = 1.638x - 1.1847]。由于纳米材料使更多抗体固定在传感表面且表面电流得到改善，在剂量依赖范围（3 - 100皮克/毫升）内实现了更低的检测限。此外，用相关生物分子进行的对照实验未显示电容变化，而在人血清中加入IL-3会增加电容，表明对IL-3的检测具有特异性和选择性。本研究通过潜在有用的方法识别和定量IL-3，并有助于诊断脓毒症发作。
