Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, School of Materials and Energy, Southwest University, Chongqing 400715, P. R. China.
Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education, Southwest University, Chongqing 400715, P. R. China.
ACS Appl Mater Interfaces. 2021 May 5;13(17):20708-20717. doi: 10.1021/acsami.1c03566. Epub 2021 Apr 26.
The marked increase in bacterial colonization of medical devices and multiple drug resistance to traditional antibiotics underline the pressing need for developing novel antibacterial surface coatings. In the present investigation, natural polyphenol tannic acid (TA)-capped silver nanoparticles (TA-Ag NPs) were synthesized via an environmentally friendly and sustainable one-step redox reaction under UV irradiation with a simultaneous and uniform deposition on polydimethylsiloxane (PDMS) and other substrate surfaces. In the synthesis process, the dihydroxyphenyl and trihydroxyphenyl groups of TA actively participate in Ag reduction, forming co-ordination linkages with Ag NPs and bestowing the deposition on the PDMS surface. The physico-chemical features of TA-Ag NPs were characterized in detail. Microscopic examination, surface elemental analysis, and wettability measurements clearly reveal the decoration of TA-Ag NPs on the substrate surfaces. The modified PDMS surfaces can kill the adhered bacteria or resist the bacterial adhesion, and no live bacteria can be found on their surfaces. Most importantly, the modified PDMS surfaces exhibit predominant antibacterial effects both in vitro in the catheter bridge model and in vivo in a rat subcutaneous infection model. On the other hand, the functionalized surfaces exhibit only a negligible level of cytotoxicity against L929 mouse fibroblasts with no side effects on the major organs of Sprague-Dawley rats after implantation, indicating their biocompatibility for potential biomedical applications.
医疗器械上细菌定植的明显增加和传统抗生素的多重耐药性，突显了开发新型抗菌表面涂层的迫切需要。在本研究中，通过在紫外光照射下进行环保且可持续的一步氧化还原反应，合成了天然多酚单宁酸（TA）包覆的银纳米粒子（TA-Ag NPs），同时均匀地沉积在聚二甲基硅氧烷（PDMS）和其他基底表面上。在合成过程中，TA 的二羟基苯基和三羟基苯基基团积极参与 Ag 的还原，与 Ag NPs 形成配位键，并赋予 PDMS 表面的沉积。详细表征了 TA-Ag NPs 的物理化学性质。显微镜检查、表面元素分析和润湿性测量清楚地表明 TA-Ag NPs 修饰在基底表面。改性后的 PDMS 表面可以杀死附着的细菌或抵抗细菌附着，表面上没有活细菌。最重要的是，改性后的 PDMS 表面在导管桥模型中的体外和皮下感染大鼠模型中的体内均表现出显著的抗菌效果。另一方面，功能化表面对 L929 小鼠成纤维细胞表现出极低的细胞毒性，且对 Sprague-Dawley 大鼠主要器官无副作用，表明其具有潜在的生物医学应用的生物相容性。
