Damoogh Sheyda, Vosough Mehrad, Hadifar Shima, Rasoli Masoumeh, Gorjipour Ali, Falsafi Sarvenaz, Behrouzi Ava
Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Science, Islamic Azad University, Tehran, Iran.
Department of Biology, Faculty of Science, Nourdanesh Institute of Higher Education, Isfahan, Iran.
BMC Res Notes. 2021 Apr 26;14(1):156. doi: 10.1186/s13104-021-05568-x.
Gut-microbiota plays key roles in many aspects like the health and illness of humans. It's well proved that modification of gut microbiota by probiotics is useful for improving inflammatory bowel disease (IBD) conditions. According to recent studies, different types of bacterial metabolites can affect immune cells and inflammation conditions. The present study aimed to evaluate the anti-inflammatory effects of metabolites of E. coli Nissle1917.
The cell-free supernatant could modulate TNF-α production and affected many crucial mediators in the Toll-like receptor (TLR) signaling pathway. Also, supernatant showed significant dose-dependent properties in this regard. In this study, the TLR signaling pathway was found among probable mechanisms by which probiotics can affect inflammatory situations. These findings provide additional evidence on the use of probiotic metabolites for inhibiting and down-regulating numerous key mediator factors in the TLR signaling pathway. Aberrant or dysfunctional TLR signaling contributes to the development of acute and chronic intestinal inflammatory pathways in IBD. Therefore, finding a component that can affect this process might be considered for therapeutic targets in IBD patients.
肠道微生物群在人类健康与疾病等诸多方面发挥着关键作用。益生菌对肠道微生物群的调节作用已被充分证明有助于改善炎症性肠病（IBD）状况。根据近期研究，不同类型的细菌代谢产物可影响免疫细胞和炎症状况。本研究旨在评估大肠杆菌Nissle1917代谢产物的抗炎作用。
无细胞上清液可调节肿瘤坏死因子-α（TNF-α）的产生，并影响Toll样受体（TLR）信号通路中的许多关键介质。此外，上清液在这方面表现出显著的剂量依赖性。在本研究中，TLR信号通路是益生菌影响炎症状况的可能机制之一。这些发现为使用益生菌代谢产物抑制和下调TLR信号通路中的众多关键介质因子提供了更多证据。异常或功能失调的TLR信号传导会导致IBD中急性和慢性肠道炎症途径的发展。因此，寻找一种能够影响这一过程的成分可能被视为IBD患者的治疗靶点。
