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基于染料组合的SARS-CoV-2比色等温核酸检测

Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination.

作者信息

Wu Shanshan, Liu Xiyang, Ye Shenglong, Liu Jianmin, Zheng Wei, Dong Xue, Yin Xiushan

机构信息

Applied Biology Laboratory, College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, 110142, Shenyang, China.

Biotech & Biomedicine Science (Shenyang) Co. Ltd, Shenyang, 110000, China.

出版信息

Heliyon. 2021 Apr;7(4):e06886. doi: 10.1016/j.heliyon.2021.e06886. Epub 2021 Apr 21.

DOI:10.1016/j.heliyon.2021.e06886
PMID:33903853
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8059943/
Abstract

RT-LAMP detection of SARS-CoV-2 has been demonstrated to be a valuable diagnostic method for the diagnosis of COVID-19, which can rapidly screen carriers of the virus to effectively control the spread of the SARS-CoV-2. Here, we present a combination of dyes for isothermal detection of SARS-CoV-2 as a commercial alternative, with expanded colorimetric spectrum. We compared them with commercial reagents and proved their suitability and sensitivity through clinical RNA samples. In addition, together with commercial single dye indicators, we believe the expanded color spectrum developed here as an indicator of rapid detection will promote the diagnosis of COVID-19.

摘要

逆转录环介导等温扩增技术（RT-LAMP）检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）已被证明是一种用于诊断冠状病毒病（COVID-19）的有价值的诊断方法，它可以快速筛查病毒携带者，以有效控制SARS-CoV-2的传播。在此，我们展示了一种用于SARS-CoV-2等温检测的染料组合，作为一种商业替代方案，具有扩展的比色光谱。我们将它们与商业试剂进行了比较，并通过临床RNA样本证明了它们的适用性和灵敏度。此外，连同商业单染料指示剂一起，我们认为这里开发的扩展色谱作为快速检测指标将促进COVID-19的诊断。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a805/8102747/d93782025621/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a805/8102747/458e7ed6a3fe/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a805/8102747/d93782025621/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a805/8102747/458e7ed6a3fe/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a805/8102747/d93782025621/gr2.jpg

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