Chen Minghai, Zhang Xian-En
CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Int J Biol Sci. 2021 Apr 10;17(6):1574-1580. doi: 10.7150/ijbs.59184. eCollection 2021.
The ongoing coronavirus disease 2019 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has posed a serious threat to global public health and social stability. There is an urgent need for understanding the nature and infection mechanism of the virus. Owing to its high infectivity and pathogenicity and lack of effective treatments, live SARS-CoV-2 has to be handled in biosafety level 3 laboratories, which has impeded research into SARS-CoV-2 and the development of vaccines and therapeutics. Pseudotyped viruses that lack certain gene sequences of the virulent virus are safer and can be investigated in biosafety level 2 laboratories, providing a useful virological tool for the study of SARS-CoV-2. In this review, we will discuss the construction of SARS-CoV-2 pseudoviruses based on different packaging systems, current applications, limitations, and further explorations.
由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起的2019年冠状病毒病大流行，对全球公共卫生和社会稳定构成了严重威胁。迫切需要了解该病毒的本质和感染机制。由于其高传染性和致病性且缺乏有效治疗方法，活的SARS-CoV-2必须在生物安全3级实验室中处理，这阻碍了对SARS-CoV-2的研究以及疫苗和治疗方法的开发。缺乏致病病毒某些基因序列的假型病毒更安全，可以在生物安全2级实验室中进行研究，为SARS-CoV-2的研究提供了有用的病毒学工具。在这篇综述中，我们将讨论基于不同包装系统的SARS-CoV-2假病毒的构建、当前应用、局限性及进一步探索。
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