含有德怀尔油树脂的局部用制剂可促进皮肤伤口愈合。

Topical formulations containing Dwyer oleoresin improve cutaneous wound healing.

作者信息

Gosuen Gonçalves Dias Fernanda, de Freitas Pereira Lucas, Andrade Furtado Ricardo, Modé Magalhães Geórgia, Pacheco Miguel Marina, Gustavo Gosuen Gonçalves Dias Luis, Torrecilhas Jorge Adriana, Dos Santos Honsho Cristiane, Ricardo Ambrósio Sérgio, Kenupp Bastos Jairo, Silva Carrijo Micaela, Crispim Tavares Denise

机构信息

Department of Veterinary Medicine, Universidade de Franca, Franca (SP), Brazil.

Department of Veterinary Medicine, Instituto Federal do Sul de Minas Gerais, Muzambinho (MG), Brazil.

出版信息

Avicenna J Phytomed. 2021 Mar-Apr;11(2):120-133.

PMID:33907671

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8051314/

Abstract

OBJECTIVE

Evaluation of the healing and toxicological effects of Dwyer oleoresin (CDO).

MATERIALS AND METHODS

Rodents with skin lesions were divided into nine groups, including daily treatments with 1, 3 and 10% CDO, collagenase, antibiotic ointment and control groups, for 14 days.

RESULTS

Treatment with 10% CDO reduced skin edema and hyperplasia, demonstrating anti-inflammatory effect of the oil. Reduction in the wound area was observed, indicating the healing effect of CDO. Histopathological analysis showed increases in angiogenesis and re-epithelialization in animals treated with the highest concentration. On the other hand, no alterations in ulcerations, inflammatory infiltrate, hemorrhage, congestion, degeneration, percentage of collagen fibers, number of cells stained with anti-macrophage migration inhibitory factor, or density of area stained with anti-collagen I and III were found. Toxicogenetic analysis revealed no differences in micronucleus frequencies or in the ratio of polychromatic erythrocytes to total erythrocytes between treated and negative control, demonstrating the absence of genotoxicity and cytotoxicity, respectively. There was no difference in levels of liver enzymes among groups, indicating the absence of hepatotoxicity.

CONCLUSION

Formulations of CDO exerted beneficial effects on the stages of cutaneous wound healing and are promising options for the treatment of wounds.

摘要

目的

评估杜松子油树脂（CDO）的愈合及毒理学效应。

材料与方法

将有皮肤损伤的啮齿动物分为九组，包括每日用1%、3%和10%的CDO、胶原酶、抗生素软膏治疗的组以及对照组，治疗14天。

结果

用10%的CDO治疗可减轻皮肤水肿和增生，表明该油具有抗炎作用。观察到伤口面积减小，表明CDO具有愈合作用。组织病理学分析显示，用最高浓度治疗的动物血管生成和再上皮化增加。另一方面，未发现溃疡、炎性浸润、出血、充血、变性、胶原纤维百分比、抗巨噬细胞迁移抑制因子染色的细胞数量或抗I型和III型胶原染色区域的密度有改变。毒理遗传学分析显示，治疗组与阴性对照组之间微核频率或多染红细胞与总红细胞的比例无差异，分别表明不存在遗传毒性和细胞毒性。各组肝酶水平无差异，表明不存在肝毒性。