Mottola Anna, Bonerba Elisabetta, Bozzo Giancarlo, Marchetti Patrizia, Celano Gaetano Vitale, Colao Valeriana, Terio Valentina, Tantillo Giuseppina, Figueras Maria José, Di Pinto Angela
Department of Veterinary Medicine, University of Bari Aldo Moro, Prov. le Casamassima, km 3, 70010 Valenzano, Bari, Italy.
Department of Veterinary Medicine, University of Bari Aldo Moro, Prov. le Casamassima, km 3, 70010 Valenzano, Bari, Italy.
Int J Food Microbiol. 2016 Nov 7;236:33-7. doi: 10.1016/j.ijfoodmicro.2016.07.012. Epub 2016 Jul 11.
Given that changes in consumer food behaviours have led to an increase in the demand for pre-cut ready-to-eat (RTE) vegetables, and that few data are currently available on the occurrence of Arcobacter spp. in such foods, the aim of the present study was to assess the occurrence of Arcobacter spp. that carry virulence-associated genes on pre-cut RTE vegetables, using cultural and molecular methods. Arcobacter was detected using biomolecular identification methods in 44/160 (27.5%) of the samples, of which 40/44 (90.9%) isolates corresponded to A. butzleri and 4/44 (9.1%) to A. cryaerophilus. Studying the incidence of 9 virulence-associated genes revealed the widespread distribution of these genes among the Arcobacter isolates tested. The results obtained in our research provided plenty of information on the health risks associated with the direct consumption of raw vegetables, and highlight the need to implement further studies at each level of the production chain, in order to obtain further information to help protect human health.
鉴于消费者食品行为的变化导致对预切即食（RTE）蔬菜的需求增加，且目前关于此类食品中弓形杆菌属的发生情况的数据很少，本研究的目的是使用培养和分子方法评估携带毒力相关基因的弓形杆菌属在预切RTE蔬菜中的发生情况。使用生物分子鉴定方法在44/160（27.5%）的样本中检测到了弓形杆菌，其中40/44（90.9%）的分离株对应于布氏弓形杆菌，4/44（9.1%）对应于嗜低温弓形杆菌。对9个毒力相关基因发生率的研究表明，这些基因在所测试的弓形杆菌分离株中广泛分布。我们研究中获得的结果提供了大量与直接食用生蔬菜相关的健康风险信息，并强调需要在生产链的每个层面开展进一步研究，以获取更多信息来帮助保护人类健康。
