Department of Physiology, College of Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dondaemun-gu, Seoul, 02447, South Korea.
Department of Life Science, Sogang University, Shinsu-dong, Seoul, 04107, South Korea.
Cell Mol Neurobiol. 2022 Oct;42(7):2427-2431. doi: 10.1007/s10571-021-01095-2. Epub 2021 Apr 28.
Ca1.2 channel phosphorylation plays an important role in regulating neuronal plasticity by action potential-dependent Ca entry. Most studies of Ca1.2 regulation by phosphorylation have been reported in heart and muscles. Here, we identified phosphorylation sites of neuronal Ca1.2 channel protein purified from rat brain using mass spectrometry. The functional characterization of these phosphorylation sites showed altered voltage-dependent biophysical properties of the channel, without affecting current density. These results show that neuronal Ca1.2 channel is regulated by phosphorylation in a complex mechanism involving multiple phosphorylation sites.
钙通道磷酸化在调节神经元可塑性方面起着重要作用，通过动作电位依赖的钙内流来实现。大多数关于钙通道磷酸化调节的研究都在心脏和肌肉中报道过。在这里，我们使用质谱法鉴定了从大鼠脑中纯化的神经元钙通道蛋白的磷酸化位点。这些磷酸化位点的功能特征表明，通道的电压依赖性生物物理特性发生了改变，而电流密度没有受到影响。这些结果表明，神经元钙通道的磷酸化调节是一种复杂的机制，涉及多个磷酸化位点。
