Melo Gessica Baptista de, Mazzaro Marcia Carolina, Gomes-Gouvêa Michele Soares, Santos Émelin Alves Dos, Souza Laura Vilela de, Elias-Oliveira Jefferson, Gryschek Ronaldo Cesar Borges, Rodrigues Rosângela Maria, Paula Fabiana Martins de
Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, Laboratório de Imunopatologia da Esquistossomose (LIM 06), São Paulo, São Paulo, Brazil.
Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo, São Paulo, São Paulo, Brazil.
Rev Inst Med Trop Sao Paulo. 2021 Apr 23;63:e32. doi: 10.1590/S1678-9946202163032. eCollection 2021.
Blastocystis sp. is an enteric protist commonly found in human fecal samples. In Brazil, few studies have been developed, but none of them has explored the presence of Blastocystis in patients with diabetes mellitus. We evaluated the occurrence and molecular identification of Blastocystis sp. among patients with diabetes mellitus in the Midwest region, Goias State, Brazil. Genomic DNA was obtained from 175 fecal samples (99 from the diabetic group and 76 from the control group). PCR was performed using pan-Blastocystis primers from the SSU-rDNA gene. Microscopic examination revealed positivity of 12.1% and 7.9% for Blastocystis in diabetics and in controls, respectively. Amplification of Blastocystis DNA was observed in 34.4% (34 of 99) and 30.3% (23 of 76) from the diabetic and control groups, respectively. Phylogenetic analyses and BLAST searches revealed six subtypes among Blastocystis isolates in the diabetic group, represented by ST1 (38.2%), ST2 (11.8%), ST3 (35.3%), ST6 (2.9%), ST7 (2.9%) and ST8 (8.8%). In the control group, ST1 (21.8%), ST2 (21.8%), ST3 (43.5%), ST6 (4.4%) and ST8 (8.7%) were identified. This study is the first report regarding the occurrence and subtypes distribution of Blastocystis in patients with diabetes mellitus in Brazil. The results reinforce the potential risk of Blastocystis infection in patients with diabetes, in addition, it contributes to the understanding of the genetic diversity of this enigmatic organism.
芽囊原虫是一种常见于人类粪便样本中的肠道原生生物。在巴西，相关研究较少，且尚无研究探讨糖尿病患者中芽囊原虫的存在情况。我们评估了巴西戈亚斯州中西部地区糖尿病患者中芽囊原虫的发生情况及分子鉴定。从175份粪便样本（99份来自糖尿病组，76份来自对照组）中提取基因组DNA。使用来自SSU - rDNA基因的泛芽囊原虫引物进行PCR。显微镜检查显示，糖尿病患者和对照组中芽囊原虫的阳性率分别为12.1%和7.9%。糖尿病组和对照组中分别有34.4%（99份中的34份）和30.3%（76份中的23份）观察到芽囊原虫DNA扩增。系统发育分析和BLAST搜索显示，糖尿病组芽囊原虫分离株中有六种亚型，分别为ST1（38.2%）、ST2（11.8%）、ST3（35.3%）、ST6（2.9%）、ST7（2.9%）和ST8（8.8%）。对照组中鉴定出ST1（21.8%）、ST2（21.8%）、ST3（43.5%）、ST6（4.4%）和ST8（8.7%）。本研究是巴西关于糖尿病患者中芽囊原虫发生情况及亚型分布的首次报告。研究结果强化了糖尿病患者感染芽囊原虫的潜在风险，此外，有助于了解这种神秘生物的遗传多样性。
