Rondón Silvia, Cavallero Serena, Link Andrés, González Camila, D'Amelio Stefano
Department of Public Health and Infectious Diseases, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
Laboratorio de Ecología de Bosques Tropicales y Primatología (LEBTYP), Departamento de Ciencias Biológicas, Universidad de los Andes, Cra. 1 N° 18a-12, Bogotá 111711, Colombia.
Pathogens. 2023 Apr 6;12(4):569. doi: 10.3390/pathogens12040569.
Infection with sp. has been reported in free-living and captive non-human primates (NHPs); however, surveys on sp. from north-western South America are scarce. This study aimed to identify sp. in free-ranging NHPs living in Colombia. A total of 212 faecal samples were collected from , , , , , and . Smears and flotation were used for morphological identification. For samples microscopically classified as positive for sp., we used conventional PCR to amplify and sequence two regions of the SSU rRNA gene and used Maximum Likelihood methods and Median Joining Network analyses for phylogenetic analyses. Via microscopy, 64 samples were sp. positive. Through molecular analyses, 18 sequences of sp. subtype 8 (ST8) were obtained. Strain and allele assignment together with a comparative phylogenetic approach confirmed that the sequences were ST8. Alleles 21, 156, and 157 were detected. Median Joining network analyses showed one highly frequent haplotype shared by specimens from Colombia and Peru and close relationships between haplotypes circulating in NHPs from Colombia, Ecuador, Brazil, and Mexico. This survey could support the elaboration of a more accurate epidemiological picture of the sp. infecting NHPs.
已有报道称，自由生活和圈养的非人灵长类动物（NHPs）感染了 种；然而，来自南美洲西北部的 种调查却很稀少。本研究旨在鉴定生活在哥伦比亚的野生非人灵长类动物中的 种。总共从 、 、 、 、 和 收集了212份粪便样本。涂片和浮选用于形态学鉴定。对于显微镜下分类为 种阳性的样本，我们使用常规PCR扩增和测序SSU rRNA基因的两个区域，并使用最大似然法和中位数连接网络分析进行系统发育分析。通过显微镜检查，64个样本为 种阳性。通过分子分析，获得了18个 种8型（ST8）序列。菌株和等位基因分配以及比较系统发育方法证实这些序列为ST8。检测到等位基因21、156和157。中位数连接网络分析显示，来自哥伦比亚和秘鲁的样本共享一种高频单倍型，并且在哥伦比亚、厄瓜多尔、巴西和墨西哥的非人灵长类动物中循环的单倍型之间存在密切关系。这项调查有助于更准确地描绘感染非人灵长类动物的 种的流行病学情况。
