Hemeg Hassan A
Department of Medical Laboratory Technology, College of Applied Sciences, Taibah University, P.O. Box 344, Al-Madinah Al-Munawarah, Saudi Arabia.
Saudi J Biol Sci. 2021 Apr;28(4):2098-2101. doi: 10.1016/j.sjbs.2021.01.017. Epub 2021 Jan 20.
Companion animals carry different microorganism of severely public health hazard for human; the kindness relation and contact between humans and companion animals may the route in the transmission of most zoonotic bacteria, including Methicillin-Resistant (MRSA). Therefore, the current study investigate the companion animals mainly dogs and cat as a reservoir for MRSA and the genetic similarity between the recovered strains of MRSA from such companion animals and their owners. One hundred swabs were collected under aseptic condition from companion animals and seventy swabs were collected from nasal and soft tissue of the infected owners in contact. All samples were examined with standard microbiological techniques, antimicrobial sensitivity, molecular typing and genetic finger printing using RAPD-PCR to determine the genetic finger printing of the recovered strains from humans and companion animals. The prevalence of the MRSA was higher in dog's swabs than human swabs. Dog swabs showed a rate of (44.4%), cat's revealed (27.3%), while the owner swabs could detect (42.8%). The antibiotics profiles were 69.2% and all MRSA strains were positive for gene (100%), while only 25 strains (38.5%) were positive for Panton Valentine Leukocidin ( gene). Phylogenetic tree revealed 4 clusters with complete genetic relatedness and higher identity between the strains recovered from humans and companion animals. Our results revealed that there is great similarity between the recovered strains, indicating that pets play an important role in colonization and transmitting MRSA to humans, and vice versa.
伴侣动物携带对人类具有严重公共卫生危害的不同微生物；人类与伴侣动物之间亲密的关系和接触可能是包括耐甲氧西林金黄色葡萄球菌（MRSA）在内的大多数人畜共患病细菌传播的途径。因此，本研究调查了主要作为MRSA宿主的伴侣动物（主要是狗和猫）以及从此类伴侣动物及其主人身上分离出的MRSA菌株之间的遗传相似性。在无菌条件下从伴侣动物身上采集了100份拭子，并从有接触的受感染主人的鼻腔和软组织采集了70份拭子。所有样本均采用标准微生物技术、抗菌药敏试验、分子分型以及使用随机扩增多态性DNA聚合酶链反应（RAPD-PCR）进行基因指纹分析，以确定从人类和伴侣动物身上分离出的菌株的基因指纹。MRSA在狗的拭子中的流行率高于人类拭子。狗的拭子显示流行率为（44.4%），猫的为（27.3%），而主人的拭子检测出率为（42.8%）。抗生素谱为69.2%，所有MRSA菌株的基因均为阳性（100%），而只有25株（38.5%）的杀白细胞素基因（基因）为阳性。系统发育树显示有4个聚类，从人类和伴侣动物身上分离出的菌株之间具有完全的遗传相关性和更高的同一性。我们的结果表明，分离出的菌株之间有很大的相似性，这表明宠物在MRSA定植并传播给人类方面发挥着重要作用，反之亦然。
