Partial purification and characterization of 2, 4-diacetylphloroglucinol producing VSMKU3054 against bacterial wilt disease of tomato.

We find out the antimicrobial potential of partially purified 2,4-diacetylphloroglucinol (DAPG) against and fungal plant pathogens isolated from tomato rhizobacterium VSMKU3054. The present study is mainly focused on the control of wilt disease of tomato by our isolate VSMKU3054 and DAPG. The cell free culture filtrate of VSMKU3054 was significantly arrested the growth of and fungal pathogens such as , , and compared to control. The existence of DAPG from the crude metabolites of VSMKU3054 was confirmed on TLC with Rf value 0.34, which is coincide with that of authentic phloroglucinol. The partially purified DAPG exhibited much higher activity against at 30 µg/ml than the fungal plant pathogens compared to control. The antimicrobial partially purified compound was identified as DAPG by UV, FT-IR and GC-MS analysis. The percentage of live cells of when supplemented with DAPG at 30 µg/ml, significantly controlled the living nature of up to 68% compared to tetracycline and universal control observed under high content screening analysis. The selected isolate VSMKU3054 and DAPG significantly controlled wilt disease of tomato up to 59.5% and 42.12% on 3rd and 7th days compared to positive and negative control by detached leaf assay. Further, in silico analysis revealed that high interaction of DAPG encoding protease with lectin which is associated with . Based on our findings, we confirmed that VSMKU3054 and DAPG could be used a potential bio inoculants for the management of bacterial wilt disease of tomato.