Sierra-Lopera Leidy Maritza, Zapata-Montoya Jose Edgar
University of Antioquia, Nutrition and Food Technology Group, 70th Street No. 52 - 21, 050010, Medellin, Antioquia, Colombia.
Biotechnol Rep (Amst). 2021 Apr 3;30:e00611. doi: 10.1016/j.btre.2021.e00611. eCollection 2021 Jun.
The objective of this study was to optimize the conditions of enzymatic hydrolysis (type of enzyme, pH, temperature (T), substrate (S) and enzyme concentration (E)) to increase content of soluble peptides (P), antioxidant activities and degree of hydrolysis DH (%), in hydrolysates. Also, the effect of scaling up from a 0.5 L to a 7.5 L reactor, was evaluated. Hydrolysis was carried out for 3 h in a 500 mL reactor, with Alcalase® 2.4 L and Flavourzyme® 500 L enzymes. A second experimental design was then developed with S and E as factors, where DH, P and antioxidant activity, were response variables. The Alcalase® 2.4 L was the most productive enzyme, with optimal S and E of 45 g/L and 4.4 g/L, respectively. Its hydrolysates showed antioxidant activities with IC50 of 0.76 g/L, 12 g/L and 8 g/L for ABTS, FRAP and ICA, respectively. The scale up didn't showed negative effect on the hydrolysis.
本研究的目的是优化酶解条件（酶的类型、pH值、温度（T）、底物（S）和酶浓度（E）），以提高水解产物中可溶性肽（P）的含量、抗氧化活性和水解度DH（%）。此外，还评估了从0.5 L反应器扩大到7.5 L反应器的效果。在500 mL反应器中使用Alcalase® 2.4 L和Flavourzyme® 500 L酶进行3小时的水解。然后以S和E为因素开展第二个实验设计，其中DH、P和抗氧化活性为响应变量。Alcalase® 2.4 L是最具生产效率的酶，其最佳S和E分别为45 g/L和4.4 g/L。其水解产物对ABTS、FRAP和ICA的抗氧化活性的IC50分别为0.76 g/L、12 g/L和8 g/L。扩大规模对水解没有负面影响。
