Biotechnology Centre, Jawaharlal Nehru Agriculture University, Jabalpur, 482004, India.
Lac Production Division, ICAR-Indian Institute of Natural Resins and Gums, Namkum, Ranchi, Jharkhand, 834010, India.
Mol Biol Rep. 2021 Apr;48(4):3097-3106. doi: 10.1007/s11033-021-06356-3. Epub 2021 Apr 28.
Several species of the Lamiaceae family are the primary source of bioactive aromatic oils and secondary metabolites, having broader applications in the cosmetics, pharmaceuticals, food, confectionery and liquor industries. Due to the scarcity of raw materials and high costs of this family's economically vital species, its products often adulterated to cater to the market's high demand. The present study provides a DNA based approach for identifying different species of this family. Henceforth, the performance of three already proposed barcode loci (matK, trnH-psbA and trnL) was examined for their PCR amplification and species recognition efficacy on various Lamiaceae species and cultivars using three different approaches such as pairwise genetic distance method, BLASTn and phylogenetic tree based on maximum likelihood (ML) analysis. Results illustrate that among all the DNA barcoding loci, matK locus can accurately and efficiently distinguish all the studied species followed by trnH-psbA and trnL. Present investigation may help diminish the illegal trade and events of adulteration of medicinally important plants species in genus Mentha, Ocimum and Plectranthus. This investigation will also help fulfil the scarcity of sequences of barcode loci of these species in the NCBI database. Apart from providing a molecular level reference for identifying processed herbal products, this technique also offers a convenient method for species identification and germplasm conservation of the Lamiaceae family.
唇形科的几个物种是生物活性芳香油和次生代谢物的主要来源，在化妆品、制药、食品、糖果和酒类行业有更广泛的应用。由于该科经济重要物种的原材料稀缺和成本高昂，其产品经常被掺假以满足市场的高需求。本研究提供了一种基于 DNA 的方法来鉴定该科的不同物种。因此，使用三种不同的方法，即成对遗传距离法、BLASTn 和基于最大似然法（ML）的系统发育树，检查了已经提出的三个条形码基因座（matK、trnH-psbA 和 trnL）在各种唇形科物种和品种上的 PCR 扩增和物种识别效果。结果表明，在所有 DNA 条形码基因座中，matK 基因座可以准确有效地区分所有研究的物种，其次是 trnH-psbA 和 trnL。本研究有助于减少薄荷属、牛至属和假马齿苋属中药用重要植物物种的非法贸易和掺假事件。本研究还将有助于满足这些物种在 NCBI 数据库中条形码基因座序列的缺乏。除了为鉴定加工草药产品提供分子水平的参考外，该技术还为唇形科植物的物种鉴定和种质保存提供了一种方便的方法。
