College of Biomass Science and Engineering and Healthy Food Evaluation Research Center, Sichuan University, Chengdu, 610065, P. R. China.
Food Funct. 2020 Nov 18;11(11):10149-10160. doi: 10.1039/d0fo01803a.
Tilapia (Oreochromis nilotica L.) skin collagen is a meritorious commercial resource to be exploited. The purpose of this study was to obtain, evaluate, and characterize tilapia skin collagen-derived antioxidant hydrolysates (TSCP). AAPH-induced erythrocyte hemolysis assay and antifatigue test in mice were implemented. It was indicated that TSCP treatment at 1 mg mL-1 could effectively attenuate AAPH-induced erythrocyte hemolysis rate from 56.35 ± 2.46% to 18.78 ± 2.48% (p < 0.01). A 2.5 mg/(10 g d) dose of TSCP intragastric administration could observably prolong the exhaustive swimming time of the loaded mice and its mechanism was multiple, including the decrease in the levels of serum lactic acid, serum urea nitrogen, and creatine kinase activity, thus improving the contents of liver and muscle glycogen and endogenous SOD activity. Five oligopeptides from the antioxidant fraction were identified as Gly-Hyp, Glu-Asp, Asp-Hyp-Gly, Glu-Pro-Pro-Phe, and Lys-Pro-Phe-Gly-Ser-Gly-Ala-Thr and then synthesized. Among them, the octapeptide exhibited the strongest antioxidant capacity. Therefore, tilapia skin-derived collagen is a meritorious edible resource for producing commercial functional foods, thus helping to scavenge radicals, protecting erythrocytes, and further resisting fatigue.
罗非鱼（Oreochromis nilotica L.）皮胶原蛋白是一种有价值的商业资源，值得开发。本研究旨在获得、评价和表征罗非鱼皮胶原蛋白衍生的抗氧化水解物（TSCP）。实施了 AAPH 诱导的红细胞溶血试验和小鼠抗疲劳试验。结果表明，TSCP 在 1mg/mL 时可有效降低 AAPH 诱导的红细胞溶血率，从 56.35±2.46%降至 18.78±2.48%（p<0.01）。2.5mg/（10g·d）TSCP 灌胃给药可明显延长负重游泳小鼠的力竭游泳时间，其作用机制多样，包括降低血清乳酸、血清尿素氮和肌酸激酶活性，从而提高肝和肌肉糖原含量和内源性 SOD 活性。从抗氧化部分鉴定出 5 种寡肽，分别为 Gly-Hyp、Glu-Asp、Asp-Hyp-Gly、Glu-Pro-Pro-Phe 和 Lys-Pro-Phe-Gly-Ser-Gly-Ala-Thr，然后进行合成。其中，八肽具有最强的抗氧化能力。因此，罗非鱼皮胶原蛋白是一种有价值的食用资源，可用于生产商业功能性食品，从而有助于清除自由基、保护红细胞，进一步抵抗疲劳。
