Department of Mechanical Engineering, National Chung-Hsing University, Taichung 40227, Taiwan.
Graduate Institute of Biomedical Engineering, National Chung-Hsing University, Taichung 40227, Taiwan.
Biosensors (Basel). 2021 Apr 7;11(4):109. doi: 10.3390/bios11040109.
This study aimed to develop simple electrochemical electrodes for the fast detection of chloride, sodium and potassium ions in human serum. A flat thin-film gold electrode was used as the detection electrode for chloride ions; a single-piece type membrane based solid-state ion-selective electrode (ISE), which was formed by covering a flat thin-film gold electrode with a mixture of 7,7,8,8-tetracyanoquinodimethane (TCNQ) and ion-selective membrane (ISM), was developed for sodium and potassium ions detection. Through cyclic voltammetry (CV) and square-wave voltammetry (SWV), the detection data can be obtained within two minutes. The linear detection ranges in the standard samples of chloride, sodium, and potassium ions were 25-200 mM, 50-200 mM, and 2-10 mM, with the average relative standard deviation (RSD) of 0.79%, 1.65%, and 0.47% and the average recovery rates of 101%, 100% and 96%, respectively. Interference experiments with Na, K, Cl, Ca, and Mg ions demonstrated that the proposed detection electrodes have good selectivity. Moreover, the proposed detection electrodes have characteristics such as the ability to be prepared under relatively simple process conditions, excellent detection sensitivity, and low RSD, and the detection linear range is suitable for the Cl, Na and K concentrations in human serum.
本研究旨在开发用于快速检测人血清中氯离子、钠离子和钾离子的简单电化学电极。使用平面薄膜金电极作为氯离子检测电极；通过在平面薄膜金电极上覆盖 7,7,8,8-四氰基对醌二甲烷（TCNQ）和离子选择性膜（ISM）混合物，开发了用于钠离子和钾离子检测的一体式膜固态离子选择性电极（ISE）。通过循环伏安法（CV）和方波伏安法（SWV），可以在两分钟内获得检测数据。氯离子、钠离子和钾离子标准样品的线性检测范围分别为 25-200mM、50-200mM 和 2-10mM，平均相对标准偏差（RSD）分别为 0.79%、1.65%和 0.47%，平均回收率分别为 101%、100%和 96%。与 Na、K、Cl、Ca 和 Mg 离子的干扰实验表明，所提出的检测电极具有良好的选择性。此外，所提出的检测电极具有能够在相对简单的工艺条件下制备、出色的检测灵敏度和低 RSD 等特点，检测线性范围适用于人血清中的 Cl、Na 和 K 浓度。
