Yoo Kyung-Hyeon, Kim Yong-Il, Yoon Seog-Young
School of Materials Science and Engineering, Pusan National University, Busan 46241, Korea.
Department of Orthodontics, Dental Research Institute, Pusan National University, Yangsan 50612, Korea.
Materials (Basel). 2021 Apr 8;14(8):1843. doi: 10.3390/ma14081843.
Calcium silicate-based cement has been widely used for endodontic repair. However, it has a long setting time and needs to shorten setting time. This study investigated the effects of magnesium (Mg) ion on the setting reaction, mechanical properties, and biological properties of calcium silicate cement (CSC). Sol-gel route was used to synthesize Mg ion-doped calcium silicate cement. Synthesized cement was formulated with the addition of different contents of Mg ion, according to 0, 1, 3, 5 mol% of Mg ion-doped calcium silicate. The synthesized cements were characterized with X-ray diffraction (XRD), Fourier transformed infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM). We also evaluated the physicochemical and biological properties of cement, such as the setting time, compressive strength, micro-hardness, simulated body fluid (SBF) immersion, cytotoxicity, and cell differentiation tests. As a result, the Mg ion improves the hydration properties of calcium silicate cement, and the setting time is reduced by increasing the amounts of Mg ion. However, the mechanical properties deteriorated with increasing Mg ion, and 1 and 3 mol% Mg-doped calcium silicate had appropriate mechanical properties. Also, the results of biological properties such as cytotoxicity, ALP activity, and ARS staining improved with Mg ion. Consequently, the optimal condition is 3 mol% of Mg ion-doped calcium silicate (3%Mg-CSC).
硅酸钙基水门汀已广泛应用于牙髓修复。然而，其凝固时间较长，需要缩短凝固时间。本研究调查了镁（Mg）离子对硅酸钙水门汀（CSC）凝固反应、力学性能和生物学性能的影响。采用溶胶-凝胶法合成镁离子掺杂的硅酸钙水门汀。根据0、1、3、5 mol%的镁离子掺杂硅酸钙，添加不同含量的镁离子来配制合成水门汀。通过X射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）和扫描电子显微镜（SEM）对合成的水门汀进行表征。我们还评估了水门汀的物理化学和生物学性能，如凝固时间、抗压强度、显微硬度、模拟体液（SBF）浸泡、细胞毒性和细胞分化试验。结果表明，镁离子改善了硅酸钙水门汀的水化性能，通过增加镁离子含量可缩短凝固时间。然而，力学性能随镁离子含量的增加而恶化，1和3 mol%的镁掺杂硅酸钙具有合适的力学性能。此外，细胞毒性、碱性磷酸酶（ALP）活性和茜素红染色等生物学性能结果随镁离子而改善。因此,最佳条件是3 mol%的镁离子掺杂硅酸钙（3%Mg-CSC）。
